7 : Molecular Diagnosis of Infectious Diseases Flashcards
(34 cards)
T or F
Nucleic Acid Amplification Techniques can overcome low sensitivity to improve the specificity of conventional phenotypic tests
True
T or F
Nucleic Acid Amplification Techniques provide early diagnosis for proper treatment with potential reduction in mortality and morbidity
True
It is a cartridge-based PCR
Cepheid GeneXpert®
It is a testing platform that is used for gastrointestinal, respiratory, and cerebrospinal organisms
Biofire® Filmarray®
It is a testing platform (microarray) that detects multiple pathogens within 1 chip and is more expensive
(e.g. : 1 chip = multiple pathogens)
Biofire® Filmarray®
It is a testing platform that uses FRET and hydrolysis probes such as quencher & reporter
Roche COBAS® TaqMan®
It is a viral loading system used to detect HIV, HBV, & HCV
Roche COBAS® TaqMan®
It is a testing platform used to detect HPV HR and uses Scorpion detection
Seegene Anyplex™ II
It is a testing platform used to detect HPV HR and uses Scorpion detection
Seegene Anyplex™ II
These are are ideal
primer targets for amplification
from all bacterial species and remain stable despite evolution
Conserved regions
In diagnosis with sequencing, measuring the degree of divergence is observed within ____ rRNA molecule
16s rRNA
In diagnosis with sequencing, the _________ sequence obtained is compared to a database containing sequences of known microorganisms
signature sequence
T or F
In diagnosis with sequencing, the number of similar nucleotide bases between sequences is used to calculate the percent identify and ascertain the identification of the microorganisms
True
T or F
The rRNA genes and their intergenic regions found in bacteria are commonly used for prokaryotic phylogenetic studies.
True
The small-subunit rRNA
molecule is a fragment
with a sedimentation
coefficient of 16S and is
encoded by a roughly
________ gene.
1542-bp gene
It known as bet subunit of RNA polymerase
rpoB
It is a DNA target known as manganese-dependent superoxide dismutase
sodA
It is a DNA target also known as elongation factor Tu
tuf
What are the common,y used OCR targets for detection of Bartonella spp. under molecular characterization of isolated from cultures?
- citrate synthase gltA
- B-subunit if RNA polymerase rpob
- 16s-23s internal transcribed spacer ITS
- 16s ribosomal RNA gene 16s
- Riboflavin synthase ribC
- NADH dehydrogenase gamma subunit nuoG
- Cell division protein fitsZ
- Heat shock protein groEL
What are the common,y used OCR targets for detection of Bartonella spp. under direct molecular detection from blood/tissues/arthropods?
- mtRNA ssrA
- 16s-23s Internal transcribes spacer ITS
- B-subunit of RNA polymerase rpoB
Recently applied techniques include NGS and proteomic (includes pathogens)
approaches is used to detect what spp?
Borellia spp.
In detecting Chlamydia trachomatis, the molecular genotyping of the gene _______ can be performed by using
restriction fragment length polymorphism on PCR products
(from: culture isolates or clinical specimens)
ompA
What are the 9 polymorphic membrane protein genes that can can be used for detecting and typing Chlamydia trachomatis?
pmpA - pmpI
The highly conserved genome of C. trachomatis cab be use
for _________ target systems.
multilocus
