7.0 Mass Transport Flashcards
(104 cards)
What type of molecule is haemoglobin?
Protein
How many polypeptide chains make up a haemoglobin molecule?
4
What does haemoglobin transport?
Oxygen
What is the primary structure of haemoglobin?
The order of amino acids in the polypeptide chains
What is the secondary structure of haemoglobin
The coiling of the polypeptide chains into a helix
What is the tertiary structure of haemoglobin?
The folding of the polypeptide chains into a precise shape
What is the quaternary structure of a haemoglobin?
The linking of the 4 polypeptide chains into an almost spherical molecule. Each polypeptide also has an associated haem group
What ion does a haem group contain?
Fe2+
How many oxygen molecules does each Fe2+ ion combine with?
1 oxygen per Fe2+
How many oxygen molecules can a molecule of haemoglobin carry in humans?
4m one per haem group, 1 haem group in a polypeptide chain, 4 polypeptide chain in a haemoglobin molecule
What is the name given to the process in which haemoglobin binds to oxygen?
Loading or associating
What is the name given to the process in which oxygen is released from a haemoglobin molecule?
Unloading or dissociating
What does affinity mean with respect to haemoglobin?
The chemical attraction between the haemoglobin and oxygen
If haemoglobin has a high affinity for oxygen, what does this mean?
Easier to associate, harder to dissociate
If haemoglobin has a low affinity, what does this mean?
Harder to associate, easier to dissociate
To be good at transporting oxygen, what 2 characteristics must haemoglobin have?
It must readily associate with oxygen where gas exchange occurs. it must readily dissociate from oxygen in respiring tissues which require it
How does haemoglobin change its affinity for oxygen?
It will change shape when in the presence of
certain molecules (e.g. carbon dioxide). If
present, the carbon dioxide changes the shape, so
the oxygen nolonger fis as well, and is easily
disassociated
What do we mean by partial pressure?
The pressure exerted by one component of a mixture of gases, if it alone occupied the volume. Eg: atmospheric pressure is 100KPa, oxygen is 21% of the atmosphere, so its partial pressure is 21KPa
What does a dissociation curve represent?
it is a graph referring to the partial pressure of oxygen, and how saturated the haemoglobin is with oxygen
What shape does the oxygen dissociation curve take?
S
Why does the oxygen dissociation curve increase slowly to begin with?
The shape of the haemoglobin molecule makes binding the first oxygen molecule difficult
Why does the oxygen dissociation curve increase sharply after a slow beginning>
The first oxygen molecule to bind changes the shape of the quaternary structure, this makes binding of subsequent molecules easier
Why does the oxygen dissociation curve increase slowly at the end of the graph?
Probability. It is easier for the molecules to bind,
but the likelyhood of the 4th oxygen molecule to
find an empty bionding site is less likely.
What is the name given to the increase in ease of binding for the other oxygen molecules?
Positive cooperativity