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Medicine COPY > Phase 1 - Week 3 (Blood) > Flashcards

Phase 1 - Week 3 (Blood) Flashcards

1
Q

List the regions of the abdomen

A
  1. Right hypochondrium (top right)
  2. Epigastric (top middle)
  3. Left hypochondrium (top left)
  4. Right lumbar (middle right)
  5. Umbilical (middle)
  6. Left lumbar (middle left)
  7. Right iliac (bottom right)
  8. Hypogastric (bottom middle)
  9. Left iliac (bottom left)
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2
Q

List the cell types that B cell precursors can mature into

A

B cells

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3
Q

When is definitive Haemostasis achieved?

A

When Fibrin formed by blood coagulation is added to the platelet mass and platelet- induced clot retraction/compaction occurs.

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4
Q

Adduction

A

Motion that pulls structure closer to the midline of the body or limb

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5
Q

Erythropoiesis

A

Production of new Erythrocytes, constant process as old RBCs are replaced

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6
Q

Describe the classificatios of Hypovolaemic Shock in terms of pulse rate

A

Class I = <100

Class II = >100

Class III = >120

Class IV = >140

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7
Q

Stages of Haemostasis

A
  1. Vasoconstriction
  2. Platelet aggregation (formation of primary platelet plug)
  3. Blood coagulation (formation of definitive fibrin clot)
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8
Q

Describe the standard precautions involved in SICP

A
  • Hand hygiene at the 5 moments
  • Care in use and disposal of sharps
  • Correct use of personal protective equipment for contact with all blood, body fluids, secretions + excretions
  • Providing care in a suitably clean environment with adequate decontaminated equipment
  • Safe disposal of waste
  • Safe management of use linen
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9
Q

Describe the mechanism of action of Haemoglobin

A

Iron combines reversibly with oxygen. The individual globin chains interact to facilitate the offloading of oxygen at lower oxygen saturations. Haemoglobin has a high affinity for oxygen in the lungs and a low affinity for oxygen in the tissues.

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10
Q

What is the main protein found in plasma and what is its function?

A

Albumin - controls osmotic pressure

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11
Q

Describe the first aid response to wounds which may contain foreign objects

A
  • Ensure airway is clear (if bleeding from mouth/nose)
  • Wear gloves to protect from infection
  • Remove clothing around wound
  • Don’t pull foreign objects out of wound - could be acting as a plug to reduce bleeding
  • Apply pressure on either side of the object
  • Lie patient down/raise legs to treat for shock (unless legs are injured)
  • Apply bandage
  • check circulation
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12
Q

What is the role of Von Willebrand’s factor in platelet function

A

Adhesion of platelets to the vascular endothelium is mediated by VWF that also carries factor VIII coagulation factor.

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13
Q

List the cell types that T cell precursors can mature into

A
  • Natural Killer Cells
  • T cells - Cytotoxic or Helper
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14
Q

Explain the cause of Shock

A

After blood loss, the drop in oxygen carrying capacity due to the decrease in RBCs means that oxygenation of tissues is not adequate to maintain normal metabolic functions

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15
Q

Explain the observations made in the Breathing stage of ATLS

A
  • Check for fractures (sternum/scapula)
  • Can have ‘flail’ - loose part of ribs due to fracture, falls in when inhaling
  • Haemothorax - collection of blood in pleural space
  • Pneumothorax - collection of air in pleural space (collapsed lung)
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16
Q

Describe the function of the primary platelet plug

A

The unstable primary haemostatic plug produced by these platelet reactions in the first minute or so following injury is usually sufficient to provide temporary control of bleeding.

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17
Q

Define differentiation

A

The process by which relatively unspecialised cells, e.g. stem cells, acquire specialised structural and/or functional features that characterise the cells, tissue or organs of the organism

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18
Q

Elevation

A

Movement in a superior direction

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19
Q

List the cell types that granulocyte CFUs can mature into

A
  • Monocytes (which mature into Macrophages or Neutrophils)
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20
Q

Explain the structure and role of the cytoskeleton in RBCs

A

Responsible for maintaining shape, proteins include spectrin, actin, ankyrin. Spectrin is most abundant consists of Alpha and Beta chains wound around each other.

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21
Q

Describe the structure of the plasma membrane of platelets.

A

Coated by glycoprotein 1b and GP2b-GP3a, involved in the attachment of platelets of platelets to Von Willebrand’s factor. Membrane is invaginated to form a system of cytoplasmic channels called the invaginated membrane system.

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22
Q

Unipotent

A

Can give rise to one specific adult body tissue cell

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23
Q

Describe the classifications of Hypovolaemic Shock in terms of respiration rate

A

Class I = 14-20

Class II = 20-30

Class III = 30-40

Class IV = >40

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24
Q

How are proteins encoded?

A

Read from mRNA in codons of 3 bases according to the genetic code

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25
Q

Explain the observations made in the Circulation stage of ATLS

A
  • Check for cardiac tamponade - bleeding between surface of heart and pericardium limiting movement of heart
  • Tension pneumothorax
  • Major haemothorax - >1l blood in chest
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26
Q

List the possible types of post-translational modifications

A
  1. Glycosylation (sugar chains added)
  2. Phosphorylation
  3. Other - hydroxylation, Prenylation, Acyl lipidation etc.
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27
Q

Describe the steps involved in the Intrinsic Pathway of blood coagulation

A
  1. Factor VII and Factor V = Factor VIIa

and Factor Va by the small amounts of thrombin generated during initiation

  1. XII = XIIa due to exposed collagen

XI = XIa due to XIIa

IX = IXa due to XIa (and calcium)

  1. Factor IXa and Factor VIIa = Intrinsic Xase
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28
Q

How can more than one protein be produced from one gene?

A

Post-translational modifications

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29
Q

Describe the steps involved in the Extrinsic Pathway of blood coagulation

A
  1. Tissue factor + plasma factor VII = Factor VIIa-Tissue factor (Extrinsic Factor Xase)
  2. Factor IX = Factor IXa

and

Factor X = Factor Xa by Extrinsic Factor Xase

  1. Factor IXa and Factor Xa convert Prothrombin = Thrombin
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30
Q

Describe the structure of erythrocytes

A
  • Non-nucleated, biconcave-shaped cells - Lack organelles - only have a plasma membrane, cytoskeleton, haemoglobin and glycolytic enzymes
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31
Q

Describe bites + treatment

A
  • Small entry wound - Possibly deep - Foreign body contamination - Animal -Human - Human blood borne viruses - Antibiotics, tetanus, vaccines - Irrigation, delayed closure?(infection)
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32
Q

How is spontaneous clotting of blood prevented in blood vessels?

A
  • Structure of the endothelial lining - smooth vessel walls. - Chemicals to prevent clotting - thrombomodulin, nitric oxide, prostacyclin
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33
Q

Describe the structure of a Haemoglobin molecule

A

2 x Alpha chains 2 x Beta Chains 4 x Haem molecules with 4 Iron atoms

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34
Q

Why must coagulation be amplified after the Extrinsic Pathway

A

Not enough thrombin is produced during extrinsic pathway to initiate fibrin polymerisation.

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35
Q

Describe the production of platelets

A

Platelets are small cytoplasmic discoid fragments derived from megakaryocytes under the control of thrompoietin, a glycoprotein produced in the kidneys and liver. Magekaryocytes develop cytoplasmic projections that become proplatelets which fragment into platelets (takes 7-10 days). Platelets bind and degrade thrombopoietin, a mechanism that regulates platelet production.

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36
Q

Multipotent

A

Can give rise to tissue specific cell type of the human body

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37
Q

Outline the 5 moments of hand hygiene

A
  1. Before touching patient
  2. Before clean/aseptic procedures
  3. After touching a patient
  4. After touching a patient’s immediate surroundings
  5. After body fluid exposure
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38
Q

Transcriptome

A

All the mRNA molecules expressed from the genome

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39
Q

Briefly explain the steps involved in the clotting cascade.

A
  1. Initiation (Extrinsic Pathway)
  2. Amplification (Intrinsic Pathway)
  3. Common pathway
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40
Q

Abduction

A

Motion that pulls structure away from the midline of the body or limb

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41
Q

List some examples of wounds

A
  1. Abrasions
  2. Degloving
  3. Incision
  4. Laceration
  5. Bites
  6. Burns
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42
Q

Describe the symptoms associated with shock

A
  • Increase in pulse
  • Decrease in blood pressure - last sign
  • Increase in respiratory rate (v. reliable measure)
  • Decrease in urine output
  • Altered mental status
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43
Q

Describe how the components of blood other than RBCs are replaced after blood loss

A

Plasma is mostly water, so water retention by kidneys helps make up lost plasma. Salts in plasma are absorbed through the digestive system. All blood proteins are synthesised in the liver.

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44
Q

Describe the role of the triage nurse

A
  • First point of contact for patients in emergency setting
  • Assess patients - type of injury or illness, its severity, symptoms, patient explanation of emergency, and vital signs - and decide who will be seen first (traffic light system)
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45
Q

Medial

A

Closer to the midline of the body

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46
Q

How is RBC replacement stimulated after blood loss?

A
  1. Peritubular cells in the kidneys recognise that the oxygen level in the blood has decreased (due to loss of RBC) and secrete the hormone Erythropoietin.
  2. Erythropoietin travels in the bloodstream to bone marrow which stimulates an increase in the activity of HSCs to produce more cells required to replace lost cells
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47
Q

Role of the Smooth ER

A

Lipid synthesis + metabolism

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48
Q

List the sources of potential infection

A
  1. Blood and other body fluids
  2. Secretions or excretions (excluding sweat)
  3. Non-intact skin
  4. Mucous membranes
  5. Equipment or items in care environment that could be contiminated
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49
Q

List the types of Personal Protective Equipment (PPE)

A
  1. Non-latex disposable gloves
  2. Disposable aprons
  3. Sterile gloves
  4. Sterile aprons
  5. Eye protection
  6. Masks and respirators
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50
Q

Describe treatment of abrasions

A
  • Clean debris (may be moved to theatre to scrub)
  • Bleeding is not usually an issue
51
Q

Describe the transition from platelet to fibrin of the Haemostatic plug

A

The fibrin component of the Haemostatic plug increases as the fused platelets completely degranulate and autolyse and after a few hours the entire Haemostatic plug is transformed into a solid mass of cross-linked fibrin.

52
Q

List the cells found in blood.

A
  • Red blood cells (erythrocytes)
  • White blood cells (leukocytes)
  • Platelets (thrombocytes)
53
Q

Explain the observations made in the Airway stage of ATLS

A
  • Check for foreign body, swelling fractures
  • Check OPA - oral/pharyngeal airway and NPA - nasal/pharyngeal airway
54
Q

Alpha granules

A

Store proteins involved in Haemostatic functions, including platelet adhesion (fibrinogen, thrombospondin, vitronutin, laminin and VWF), blood coagulation (plasminogen), growth factors for endothelial cell repair and microbial proteins

55
Q

Describe lacerations + treatment

A
  • Due to blunt force trauma only
  • Skin splitting, tissue bridging, irregular depths/edges
  • Irrigate/clean
  • Close - glue/steristrips/staples/stitches
56
Q

Describe the cell types that can be produced from:

a) Eosinophil CFUs
b) Basophil CFUs
c) Megakaryocyte CFUs
d) Erythroid CFUs

A

a) Eosinophils
b) Basophils
c) Megakaryocytes which mature into platelets
d) Red Blood cells (Erythrocytes)

57
Q

Describe the effects of blood loss on the body

A
  • All components of blood are lost equally
  • Oxygen carry capacity of blood drops - causes shock
  • Clotting ability drops
58
Q

Describe the major planes used in anatomy

A
  1. Transverse = divides body into superior and inferior structures
  2. Sagittal = divides body into left and right structures
  3. Coronal = divides body into anterior and posterior structures
59
Q

Describe the function of erythrocytes

A

Transport of respiratory gases - oxygen/carbon dioxide. Oxygen goes from lungs to tissues and is then exchanged for carbon dioxide.

60
Q

List the major groups of Haematopoietic Growth Factors

A
  1. Colony stimulating factors
  2. Erythropoietin and Thrombopoietin
  3. Cytokines
61
Q

Lysosomal granules

A

Containing hydrolytic enzymes participating in the dissolution of thrombi

62
Q

Deep

A

Further from the surface of the body

63
Q

Fibrinolysis

A

The process that prevents clots from growing and becoming problematic

64
Q

Explain how the structure of erythrocytes makes them suited to their function

A
  • No nucleus means reduced weight and space for haemoglobin
  • Biconcave disc for flexibility
  • Membrane structure gives ability to withstand stress of turbulent circulation
65
Q

Dorsal

A

Structures derived from the back of the embryo

66
Q

Eversion

A

Movements that tilt the sole of the foot away from the midline of the body

67
Q

How does Plasmin prevent growth of thrombi at the site of injury?

A

Plasmin is capable of digesting fibrinogen, fibrin, factors V and VIII etc.

68
Q

List the divisions in lineage which can result from Haematopoietic Stem Cells

A
  1. Lymphoid stem cells - mature into T cell precursors and B cell precursors
  2. Myeloid Stem Cells - mature into Granulocyte CFUs, Eosinophil CFUs, Basophil CFUs, Megakaryocyte CFUs and Erythroid CFUs
69
Q

How many amino acids are there?

A

20

70
Q

Anterior

A

Front/closer to the front

71
Q

Supination

A

Rotation of the forearm/foot so that the palm or sole is facing anteriorly

72
Q

Describe the process of Erythropoiesis

A

Erythroid CFU –> Proerythroblasts –> Basophilic erythroblast –> Polychromatophilic erythroblast –> Orthochromatic erythroblasts –> Reticulocyte –> Erythrocyte

73
Q

Describe the classifications of Hypovolaemic Shock in terms of total % blood loss

A

Class I = up to 15% Class II = 15-30% Class III = 30-40% Class IV = >40%

74
Q

Cranial

A

Structures close to the top of the body

75
Q

Describe the stages of ATLS

A

Advanced Trauma Life Support:

A - Airway with cervical spine control (risk of broken neck)

B - Breathing with oxygenation

C - Circulation with haemorrhage control

D - Disability (neurological)

E - Exposure (prevent cold/expose more injuries)

76
Q

Plasma

A
  • Fluid component of blood - Contains salts and organic compounds (including amino acids, lipids, vitamins, proteins and hormones)
77
Q

Describe the classifications of Hypovolaemic Shock in terms of urine output

A

Class I = >30 mls/hr Class II = 20-30 mls/hr Class III = 5-15 mls/hr Class IV = negligible

78
Q

Explain how Thrombin converts Fibrinogen to cross-linked Fibrin.

A

Thrombin hydrolyses Fibrinogen releasing Fibrinopeptides A and B to form Fibrin monomers. Fibrin monomers link spontaneously by hydrogen bonds to form a loose insoluble fibrin polymer. Factor XII is also activated by thrombin and stabilises the Fibrin polymers with the formation of covalent bond cross-links.

79
Q

Describe the classifications of Hypovolaemic Shock in terms of mental status

A

Class I = Normal Class II = Mild anxiety Class III = Anxious Class IV = Confused

80
Q

Pluripotent

A

Can give rise to any type of cell in the adult human body

81
Q

Dense core granules

A

Containing mediators of vascular tone (serotonin, ADP, phosphate)

82
Q

List the populations of cells found in bone barrow

A
  1. Haematopoietic stem cells capable of self-renewal 2. Committed precursor cells responsible for the generation of distinct cell lineages 3. Maturing cells, resulting from the differentiation of the committed precursor cell population
83
Q

Describe the steps involved in the Common Pathway of blood coagulation

A
  1. Extrinsic + Intrinsic Xase activate Factor X = Factor Xa
  2. Factor Xa + Va + PL (phospholipid) + calcium = Prothrombinase complex
  3. Promthrombinase activates Prothrombin = Thrombin
  4. Thrombin converts Fibrinogen = Cross linked Fibrin Thrombin activates Factor XIII = Factor XIIIa which also contributes to the Fibrinogen = Fibrin reaction
84
Q

What is the role of Vitamin K in blood coagulation?

A

The activity of factors II, VII, IX and X is dependent on Vitamin K which is responsible for carboxylation of a number of terminal glutamic acid residues on the molecules.

85
Q

List the types of Shock

A
  1. Hypovolaemic - Haemorrhagic (external/internal) - loss of blood from intravascular space 2. Cardiogenic - Pump failure 3. Septic - Fluid redistribution - leaking capillaries, vasodilation 4. Spinal/neuro - Altered/loss of vascular tone 5. Anaphlylactic - Combination (fluid leaking/cardiac failure etc.)
86
Q

Proteome

A

All the proteins that can be expressed by an organism

87
Q

Role of the invaginated system in platelets

A

Allows for the absorption of clotting factors and the release of secretory products stored in granules in thrombin-activated platelets

88
Q

Describe the structure of the periphery of platelets

A

Hyalomere - contains microtubules and microfilaments that regulate platelet shape change, motility towards the site of injury and infection and release of granule contents.

89
Q

Describe degloving wounds

A
  • Skin/blood supply torn off - Major trauma - Commonly limbs/digits
90
Q

Describe the structure of a RBC’s membrane

A

Made of collapsible lattice of specialised proteins (cytoskeleton) and outer lipid bilayer. Lipid bilayer provides hydrophobic skin, consists mainly of a mixture of phospholipids and cholesterol.

91
Q

Haematocrit

A

Number of RBC in blood (normal = 40%)

92
Q

Compare adult (tissue) and embryonic stem cells

A

Adult (tissue) -Multipotent or single type - Able to become specialised cell types within residing tissue or specific cell type of the tissue - Useful for transplant - Difficult to culture + expand - Less controversial ethically Embryonic - Pluripotent - Able to become any cell type in body - Rejection problem - Easier to culture + expand - Extremely controversial - any embryo has potential to grow into baby

93
Q

Flexion

A

Movement that decreases the angle between body parts

94
Q

Inversion

A

Movements that tilt the sole of the foot away from the midline of the body

95
Q

Distal

A

Further from the centre of the body

96
Q

Depression

A

Movement in an inferior direction

97
Q

Describe the life-cycle of erythrocytes

A
  • RBCs circulate for 120 days - Senescent RBCs are removed by phagocytosis or destroyed by haemolysis in the spleen - RBCs are replaced in the circulation by reticulocytes which complete haemoglobin synthesis and maturation 1-2 days after entering the circulation - Reticulocytes are produced by haemopoietic tissue in red bone marrow - Before release from bone marrow RBCs shed their nuclei
98
Q

Describe the classifications of Hypovolaemic Shock in terms of blood pressure

A

Class I = Normal Class II = Normal Class III = Decreased Class IV = Decreased

99
Q

Lateral

A

Further from the midline of the body

100
Q

Superior

A

Above another part of the body

101
Q

How is blood coagulation initiated?

A

After vascular injury, when the membrane-bound Tissue factor is exposed and activated.

102
Q

Caudal

A

Structures close to the bottom of the body

103
Q

Proximal

A

Closer to the centre of the body

104
Q

Role of the Rough ER

A

Protein secretion

105
Q

Where are the cellular components of blood produced?

A

In bone marrow by Haematopoietic stem cells

106
Q

Ventral

A

Structures derived from the front of the embryo

107
Q

Describe the process of primary platelet plug formation

A
  1. Break in endothelial lining
  2. Initial adherance of platelets (via glycoprotein receptors - GP1A/GP1B - on platelet surface which interact with the extracellular matrix) to exposed connective tissue mediated by VWF
  3. Collagen exposure and thrombin generated through activation of Tissue Factor produced at the site of injury cause the adherent platelets to release their granule contents and activate platelet prostaglandin synthesis leading to the formation of TXA2 (causes further vasoconstriction)
  4. Released ADP from granules causes platelets to swell and aggregate
  5. Platelets roll over exposed VWF in direction of blood flow and GP11a/GP111b receptors become activated, resulting in firmer binding of platelets. Additional platelets from circulating blood are drawn to the area of injury.
  6. Continuing platelet aggregation promotes the growth of the Haemostatic plug, which soon covers the exposed connective tissue
108
Q

Pronation

A

Rotation of the forearm/foot so that the palm or sole is facing posteriorly

109
Q

Explain the events of the vasoconstriction phase of haemostasis

A
  • Immediate vasoconstriction of injured vessel and reflex constriction of adjacent small arteries/aterioles responsible for initial slowing of blood flow in injured area to prevent blood loss
  • Reduced blood flow allows contact activation of platelets and coagulation
  • Vasoactive amines TXA2 (thromboxane A2) serotonin etc. liberated from adherent platelets releasing their granule contents during platelet aggregation have vasoconstrictive activity
  • Involves contraction of smooth muscle cells in BVs
  • Becomes more effective as damage increases, more effective in small BVs
110
Q

Describe the classifications of Hypovolaemic Shock in terms of blood loss (ml)

A

Class I = up to 750 Class II = 750-1500 Class III = 1500-2000 Class IV = >2000

111
Q

Genome

A

All genes an organisms an organism posesses

112
Q

Describe the structure of the central region of platelets

A

Granulomere - contains mitochondria, rough endoplasmic reticulum (RER), the Golgi apparatus and 3 distinct types of granules

113
Q

Describe the components of blood in terms of percentages.

A

43-48% - Cells 52-57% - Plasma

114
Q

Inferior

A

Below another part of the body

115
Q

Posterior

A

Back/closer to the back

116
Q

Describe incisions + treatment

A
  • Sharp/penetrating - Stab (deep not wide)/slash - Look less dramatic but v. deep - Must investigate for underlying damage - Can loss blood into chest, abdomen, pelvis, long bones
117
Q

Extension

A

Movement that increases the angle between body parts

118
Q

What are the functions of blood?

A
  • Carries oxygen from lungs to tissue - Carries carbon dioxide from tissues to lungs - Clotting - Healing - Contains cells which fight infection
119
Q

Describe the process of fibrinolysis

A

Plasminogen = Plasmin by activators either from the vessel wall (Intrinsic activation) or from the tissues - extrinsic activation

120
Q

Define Standard Infection Control Precautions (SICP)

A

Used by all staff, in all care settings, at all times, for all pateints/persons whether infection is known to be present or not to ensure the safety of those being cared for, staff and visitors in the care environment

121
Q

Superficial

A

Closer to the surface of the body

122
Q

List the granules found in platelets

A
  1. Alpha granules
  2. Dense core granules
  3. Lysosomal granules
123
Q

Totipotent

A

Can give rise to embryonic membrane - any type of cell in the adult human body

Medicine COPY (18 decks)

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