Proteins-Primary Flashcards
1
Q
Proteins expressed at high levels
A
Collagen, hemoglobin
2
Q
Proteins expressed at low levels
A
Hormones, signaling proteins
3
Q
Purification and analysis by charge
A
Ion exchange chromatography,
Isoelectric focusing,
Electrophoresis
4
Q
Protein purification and analysis by Size
A
Dialysis and ultracentrifugation,
Gel electrophoresis,
Gel filtration(size exclusion)
SDS-page
5
Q
By Charge and Size
A
2D gel electrophoresis
6
Q
Protein purification and Analysis by Specificity
A
Affinity Chromatography
7
Q
Protein Purification and Analysis by Polarity
A
Paper chromatography,
Reverse-phase chromatography,
Hydrophobic chromatography
8
Q
Liquid Chromatography
A
Uses buffer or solvent
9
Q
Edman uses
A
Enzymes
10
Q
Mass spec advantages
A
Specific Sensitive Identify several peptides from a protein Multiple proteins at a time in parallel Type of modification and exact location
