7.1 PCR

Question 1

explain what is meant by the term genome

Answer 1

idk

Question 2

DNA profiling

Answer 2

compares introns and exons within DNA

Question 3

steps of DNA profiling

Answer 3

1) DNA sample is extracted
2) DNA sample is magnified using PCR
3) fluorescent tags are added
4) DNA fragments are separated using gel electrophoresis
5) gel is viewed under UV light
(1-2 is PCR and 3-5 is gel electrophoresis)

Question 4

step 1)

Answer 4

DNA heated to 94-96 degrees
H bonds between strands break
DNA separates into two stands

Question 5

Step 2)

Answer 5

mixture is cooled to 50-65 degrees
allows primers to anneal/attach to the 3’ end of the stand

Question 6

step 3)

Answer 6

mixture is heated to 72 degrees
heat tolerant DNA polymerase replicates the region of DNA
takes longer for the polymerisation of nucleotides

Question 7

step 4)

Answer 7

repeated cycles of heating and cooling to amplify this region of DNA by a thermal cycle ( aprox 30 times)

Question 8

what does PCR stand fro

Answer 8

(DNA) polymerase chain reaction)