OxPhos

Question 1

Where does OxPhos happen?

Answer 1

In the IMM, a secondary membrane in the mitochondria that forms cristae. The enzymes are imbedded in the membrane

Question 2

Where does the mitochondria come from?

Answer 2

The idea is that the mitochondria arrose from endocytosis of a cyanobacteria, as theyposses their own genome, membranes, own prot synthesis mechanism that ressembles prokaryotic, uses fMet (as prokaryotes do), and are affected by several Abx

Question 3

What is the 2 steps of OxPhos

Answer 3

Well-Ox-transfer of electrons of NADH or FADH2 to O2, translocating protons across the membrane. Phos-the PMF is transolcated back, using the energy to phosphorylate ADP to ATP

Question 4

So what are the basic enzymes of OxPhos?

Answer 4

NADH Dehydrogenase (complex i), Ubiquinone dehydrogenase (Complex III), cytochrome C oxidase (Complex IV) and succinate dehydrogenase (Complex II)-and carriers_Ubq and CytC

Question 5

What are the steps of the Ox part of the ETC?

Answer 5

When NADH transfer electrons to CI, which transfers to Ubq, then CIII, then CytC, then CcO, which finally transfers 2e to o2 making H2O - uses the basis REDOX potential

Question 6

What is the enzyme of the Phos?

Answer 6

F1F0 ATP synthase-made of the F0 is in the membrane, made of the a and c parts (electrons pass through), mouvement is passed down by gamma stalk to alpha and beta subunits (F1) which make ATP