BI203 Lecture 24: Apoptosis (Slides 24 - 38) Flashcards
Regulation of programmed cell death is mediated by integrated signaling pathways; some induce cell death and others promote cell survival.
___ pathways are activated by DNA damage and other cell stress.
___ pathways are activated by signals from other cells.
intrinsic, extrinsic
The 6 main types of BH-3 only proteins:
Bad, Bim, Bid, PUMA, Noxa, Bmf
___ and ___ are activated by the loss of growth factor signaling.
Bad, Bim
___ is activated by the activation of “Death Receptors”.
Bid
___ and ___ are activated by “DNA damage”.
PUMA, Noxa
___ is activated by the loss of cell adhesion.
Bmf
Bad and Bim are activated by the loss of ___ ___ ___.
growth factor signaling
Bid is activated by the activation of ___ ___.
Death Receptors
PUMA and Noxa are activated by ___ ___.
DNA damage
Bmf is activated by the loss of ___ ___.
cell adhesion
A major signaling pathway that promotes cell SURVIVAL is initiated by ___, which phosphorylates ___ to ___, which activates the serine/ threonine kinase ___.
___ then phosphorylates the BH3-only protein Bad in addition to FOXO transcription factors, keeping them inactive and keeping the cell alive.
PI 3-kinase, PIP2, PIP3, Akt
Polypeptides in the ___ ___ ___ (TNF) family are the death signals.
Receptors activate an initiator caspase, ___. This initiator caspase then activates effector caspases as well as the BH3-only protein ___, which leads to activation of Caspase-9.
tumor necrosis factor (TNF), Caspase-8, Bid
If DNA damage is severe, then p53 stabilization is prolonged and sustained.
Then, high levels of p53 activates transcription of genes encoding the pro-apoptotic BH3-only proteins ___ and ___, leading to cell death.
PUMA, Noxa
