Antibody Identification

Question 1

Which 5 blood group systems exhibit dosage?

Answer 1

Rh (except D)
Kidd 
Duffy
MNSs
Lutheran

Question 2

Antibodies that show dosage…

Answer 2

React more strongly with cells that have homozygous expression vs. Homozygous expression.

Question 3

What D genotype combinations are acceptable for a set of screen cells?

Answer 3

Screening cells are comprised of two cells expressing (R1R1 and R2R2) or three cells expressing (R1R1, R2R2, rr).

Question 4

When performing an antibody screen or identification, all negative tests must have what added to confirm the validity of the negative result?

Answer 4

Check cells or Coomb’s control cells

Question 5

How does the antiglobulin (AHG) reagent contribute to the agglutination reaction?

Answer 5

If the RBCs are coated with IgG antibodies, the anti-IgG antibody in the AHG reagent will create a bridge between sensitized reagent RBCs, resulting in observable agglutination.