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Clinical Microbiology > Misc GN Orgs- Needy GNB > Flashcards

Misc GN Orgs- Needy GNB Flashcards

1
Q

What is Brucella “needy”?

A

It has an extended incubation time of three weeks

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2
Q

What is Campylobacter “needy”?

A

Microaerophilic and 42 degrees

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3
Q

What is Helicobacter “needy”?

A

Doesn’t grow well
Microaerophilic
Usually urine test

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4
Q

What is Vibrio “needy”?

A

Most are halophilic

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5
Q

What is Legionella “needy”?

A

Requires L-cysteine and iron for growth

can not survive in dry environments

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6
Q

Bordetella

A

Requires special media:

Bordet-Gengou or Regan-Lowe for 7-10 days

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7
Q

What is Francisella “needy”?

A

Requires special agar:

Blood cysteine glucose agar with thiamine

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8
Q

List the Needy GNB

A 
Brucella
Campylobacter
Helicobacter
Vibrio
Legionella
Bordetella
Francisella
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9
Q

What is Brucella’s air status?

A

Aerobic

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10
Q

Brucella gram stain?

A

GNCB

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11
Q

What disease doese Brucella cause

A

zoonosis
Brucellosis or Undulant fever
–recuring fever that rises and falls

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12
Q

Brucella is acquired by

A

Ingestion of contaminated dairy
Inhalation of aerosolized particles
Abrasion from handling an infected animal
Can be a lab acquired infection

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13
Q

Where is Brucella most commonly isolated from?

A

human blood

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14
Q

What level safety hood is required for Brucella?

A

Level 3

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15
Q

Campylobacter jejuni gram stain?

A

Motile, curved GNB

very faint staining and seagull wings

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16
Q

Campylobacter jejuni air status?

A

Microaerophilic–85% N, 5% O, 10% CO2

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17
Q

What is the MCC of bacterial diarrheal disease in the US?

A

Campylobacter jejuni

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18
Q

What temp does Campylobacter jejuni grow at?

A

42 C

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19
Q

Campylobacter jejuni is oxidase

A

positive

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20
Q

Helicobacter pylori is urease

A

strongly positive

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21
Q

What disease does Helicobacter pylori cause?

A

peptic ulcers and it is the only organism that can survive in the stomach

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22
Q

What is the only organism that can survive in the stomach?

A

Helicobacter pylori

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23
Q

How is Helicobacter pylori infection MC ided?

A

via gastric biopsy or urea test; Clo Test

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24
Q

Gram stain of Helicobacter pylori?

A

Rarely done; similar to Campy, but can have U shape

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25
Q

What are Helicobacter pylori growth requirements??

A

similar to CAmpylobacter

26
Q

Vibrio cholera disease

A

rice water stools due to potent enterotoxin

27
Q

Vibrio cholera gram stain

A

“comma” shaped

28
Q

Vibrio cholera environment?

A

Natural inhabitant of salt water

29
Q

Vibrio cholera is halophilic

A

It needs salt to grow and this is why it is needy

30
Q

Vibrio cholera acquired by

A

Contaminated water or seafood

31
Q

Strains of Vibrio other than Vibrio cholera cause what disease MC?

A

wound infections

32
Q

What special agar is recommended for isolation?

A

TCBS agar

33
Q

Does Vibrio cholera grow on BAP?

A

Yes and is sometimes beta hemolytic

34
Q

What is the appearance of Vibrio cholera on TCBS agar?

A

Yellow colonies b/c it is a sucrose fermenter

35
Q

Legionella pneumophilia causes?

A

Legionairre’s disease

36
Q

Legionella pneumophilia is MC acquired by?

A

via inhalation from water systems such as air conditioners or plumbing systems

37
Q

How is Legionella pneumophilia transmitted?

A

only from contaminated water inhalation

No person to person transmission

38
Q

Media of choice for Legionella pneumophilia?

A

Buffered charcol yeast extract (BCYE)

39
Q

Does Legionella pneumophilia grow on regular media?

A

No.

Must have L-cysteine

40
Q

What are the risk for acquiring Legionella pneumophilia infection?

A

Older male smoker

41
Q

What tests other than plate growth is used for iding Legionella pneumophilia?

A

Urine antigen test

DFA

42
Q

What test is best for detecting Bordetella pertussis?

A

PCR

43
Q

What special media is required for Bordetella pertussis?

A

Bordet-Gengou or Regan-Lowe

44
Q

Gram stain Bordetella pertussis?

A

faint, tiny GNCB

45
Q

What is the optimal specimen for Bordetella pertussis?

A

Nasopharyngeal

46
Q

How long is incubation for Bordetella pertussis?

A

7 to 20 day incubation

47
Q

Virulence factors Bordetella pertussis?

A

capsule
endotoxin
pertussis toxin

48
Q

What disease is caused by Bordetella pertussis?

A

whooping cough

49
Q

Bordetella pertussis has an strong affinity for what cells?

A

ciliated epithelial cells in the mucous membranes of the respiratory tract

50
Q

How contagious is Bordetella pertussis?

A

VERY

51
Q

How can Bordetella pertussis infection be prevented?

A

Vaccine! boosters at 5 and 11

52
Q

Gram stain for Francisella tularemia?

A

Pleomorphic GNB

Very faint staining

53
Q

What disease does Francisella tularemia cause?

A

Tularemia

54
Q

What are the S/S of Tularemia?

A

Ulcer forms are the site of infection and stays for months

fever, chills, lymphoadenopathy

55
Q

How is Francisella tularemia acquired?

A

transmitted by biting flies and ticks

56
Q

Why is Francisella tularemia dangerous?

A

CAN CAUSE LAB ACQUIRED INFECTION and

BIOTERRISM

57
Q

What virulence factors does Francisella tularemia have?

A

Capsule

58
Q

Francisella tularemia oxidase reaction?

A

Negative

59
Q

Francisella tularemia catalase reaction?

A

Catalase Positive

60
Q

What is the special agar for Francisella tularemia?

A

Blood cysteine glucose agar with thiamine

61
Q

Will Francisella tularemia grow on BAP or Choc?

A

On both, but will be very tiny and faint staining

Clinical Microbiology (13 decks)

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