Microscopes Flashcards
Memories differences between TEM and SEM, advantages and disadvantages and how each microscope works.
1
Q
How do light microscopes work?
A
They use a series of glass lenses to magnify an image
2
Q
How do electron microscopes work?
A
They use a beam of electrons instead of light
3
Q
What are some advantages of light microscopes?
A
- Cheap
- Portable
- Produces colour images
- Different stains highlight different features
- Can sometimes use live materials
4
Q
What are some disadvantages of light microscopes?
A
- Low resolution
- The material has to be transparent
- Max magnification is 1000 x to 1500 x
5
Q
What are some advantages of electron microscopes?
A
- High resolution
- High magnification (200 000 x)
- Can produce 3D images
6
Q
What are some disadvantages of electron microscopes?
A
- Expensive
- Large and difficult to move
- Uses highly toxic chemicals
- Time-consuming and requires skill
- Produces black and white images
- Samples must be dead
7
Q
What does SEM stand for?
A
Scanning Electron Microscope
8
Q
What does TEM stand for?
A
Transmission Electron Microscope
