Protists Flashcards

1
Q

What are protists?

A
  • A very diverse group
  • Basically everything that is a eukaryote but not a land plant, fungi or animal
  • Most are very small
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2
Q

Who discovered protists?

A

Leeuwenhoek looking down early microscope:

» “No more pleasant sight has met my eye than this, of so many thousands of living creatures in one small drop of water”

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3
Q

Are protists uni or multicellular?

A

Most unicellular, some form colonies, and simple multicellular forms

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4
Q

How do Protists get their nutrition and reproduce?

A
  • Most nutritionally diverse of all eukaryotes, include:
    1. Photoautotrophs, which contain chloroplasts
    2. Heterotrophs, which absorb organic molecules or ingest larger food particles
    3. Mixotrophs, which combine photosynthesis and heterotrophic nutrition
  • Can reproduce asexually or sexually
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5
Q

What are the four super groups of protist phylogeny?

A

» Excavata
» SAR
» Archaeplastida
» Unikonta

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6
Q

What is clade excavata?

A
  • Monophyletic group
  • Some have an “excavated” feeding groove
  • Can be predatory heterotrophs, photosynthetic autotrophs, mixotrophs, and parasites
  • Example: Giardia intestinalis
    » Flagellated unicellular eukaryote
    » Parasitic in human intestine
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7
Q

What is Giardia?

A
  • A parasitic protist from the excavata clade:
  • shed in faeces
  • Commonly transmitted in contaminated water
  • Giardia has protective outer shell → can survive outside the body for long periods + is resistant to chlorine disinfection
    » ≈2% adults, 6-8% children in developed countries
    » Nearly 33% people in developing countries have had giardiasis
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8
Q

What are the symptoms of Giardia?

A
  • Symptoms appear around 1-3 weeks after infection, last 1-2 weeks or longer
  • Acute symptoms include
    » Diarrhea
    » Gas
    » Greasy stools that tend to float
    » Stomach or abdominal cramps
    » Upset stomach or nausea/vomiting
    » Dehydration (loss of fluids)
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9
Q

What is the SAR Clade?

A
  • Diverse monophyletic group of protists defined by DNA similarities
  • divided into three main groups:
    1) Stramenopiles
    2) Alveolates
    3) Rhizarians
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10
Q

What are Stramenophiles?

A
  • A main group of the SAR Clade
  • Key organism in many environments
    » Diatoms, brown algae and several pathogens
  • Diatoms are a key group of unicellular photosynthetic algae
    » Highly diverse (≈100,000 species)
    » Glass-like wall made of silicon dioxide (protection from predation)
    » Yellow/brown due to brown plastids
    » Usually reproduce asexually
    » Store food in a form of oil, which make cells buoyant
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11
Q

What are Diatoms?

A
  • A branch of the Stramenophiles group in the SAR Clade
  • Important component of plankton (free floating microorganism in marine and freshwater)
  • Important source of fixed carbon
    (20% of global carbon fixation) and 40% of marine primary productivity
  • Boom and bust population cycles
    » Boom when conditions favourable (lots of nutrients, low predation)
    » Then fall to the ocean floor, then when new nutrient upwelling, boom again
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12
Q

What is brown algae

A
  • A branch of the Stramenophiles group in the SAR Clade
  • » All multicellular and mostly in marine
    » Largest and most complex algae
    » Have chlorophyll-a, chlorophyll-c & carotenoid pigments
    » Store carbohydrate food reserves in the form of laminarin
    » Cell walls consist of cellulose and lignin
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13
Q

What are Alveolates?

A
  • A main group of the SAR Clade

- Membrane-enclosed sac (“alveoli”) under the plasma membrane

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14
Q

What are dinoflagellates?

A
  • A branch of the Alveolates group in the SAR Clade
  • Have flagella, and often encased in “armour plates”
  • Important in phytoplankton (small marine photosynthetic organisms)
  • Can have carotenoids as pigments giving rise to “red tides” when population blooms
    » Some produce toxins, and can contaminate (and kill) invertebrates and fish (and humans who consume them)
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15
Q

What are Apicomplexans?

A
  • A branch of the Alveolates group in the SAR Clade
  • Nearly all are parasites of animals
  • Non-photosynthetic
    » Although they have retained plastid
  • Example:
    » Plasmodium and malaria
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16
Q

What are the symptoms of plasmodium and malaria

A
  • Symptoms appear 1-2 weeks after being bitten by mosquito carrying Plasmodium
  • Symptoms include fever, headache, chills, muscle and joint pain, diarrhea, vomiting, confusion … if not treated can progress to severe illness, often leading to death
17
Q

What can be done to control malaria

A

Controlling malaria incl. vector control: insecticide-treated mosquito nets, indoor residual spraying and outdoor spraying

18
Q

What is Archaeplastida?

A
  • Third protist subgroup: Archaeplastida

- Red and green algae (protists), but also include land plants

19
Q

What is Rhodophyta (red algae)

A
  • A branch of Archaeplastida
  • Multicellular organisms with moderately large body and distinctive structure
  • Nearly all marine
  • Possess chlorophyll-a and red accessory pigment (phycoerythrin)
    » Shallow = greenish; intermediate = red; deepest = black
  • Occurs deeper than any other photosynthetic organisms (>250m)
20
Q

What is Chlorophyta (green algae)

A
  • A branch of Archaeplastida
  • Most plant-like of all algae
  • Chlorophyll-a and chlorophyll-b (no others)
  • Most live in freshwater
  • Various shapes:
    » Some unicellular and form green scum in water
    » Others filamentous
    » Many others form two dimensional sheet (thallus) such as sea lettuce (Ulva)
    » Others have complex three-dimensional structures like “true” plants
  • Some eaten for food (eg Ulva in Japan)
  • Studied intensively for bio-technology
21
Q

What is Unikonta?

A

Includes protists that are closely related to fungi and animals, as well as fungi and animals

22
Q

What are Amoebozoans

A
  • A branch of the Unikonta Clade
  • Slime moulds (once thoughts to be fungi)
    » 2 main branches: plasmodial slime moulds and cellular slime moulds
23
Q

What are Plasmodial Slime moulds?

A

» All heterotrophs and many brightly pigmented
» Feeding stage of lifecycle consisting of amoeboid mass engulfs food by phagocytosis
» Live in moist soils, leaf mulch, rotting logs
» When stressed (lack of food, drying) ceases growth, forms sexually reproductive structures called fruiting bodies or sporangia

24
Q

What are symbiotic protists?

A

Protists play key roles in ecological communities
- Some form symbiotic relationships (one or both species benefit from interaction)
- Can be mutualism where both benefit
» Eg, coral rely on photosynthetic protists called zooxanthellae, a type of dinoflagellate; protist benefit from protected living space

25
Q

What is an example of a photosynthetic protist

A

Phytoplankton ≈30% all photosynthesis – includes diatoms, dinoflagellates, multicellular algae and other aquatic protists

26
Q

What are some examples of parasitic Protists?

A
  • Malaria-causing Plasmodium
  • Dinoflagellate parasites (Pfiesteria sp.) can cause massive fish kills
  • Stramenophile Phytophthora cinnamoni is a major threat to forest of Western and South Australia
  • Phytophtora infestans caused potato late blight (→ Irish famine of the 19th century – 1 million dead and many forced to leave)