Mesenchymal Stem Cells

Question 1

What must Mesenchymal Stem cells express to be classified as them?

Answer 1

CD105, CD73, CD90

Question 2

What must MSC be able to differentiate into?

Answer 2

Osteoblasts, adipocytes, chondrocytes, osteocytes

Question 3

How do MSC respond to the environment?

Answer 3

Express receptors that detect cytokines - TNFR1, TNFR2, IFNy, IL6R
Bacterial products cause MSC to pro inflammatory phenotype —> release IL6, IL6, IFNy, MIF, GM-CSF
Increase neutrophil activation and reduce neutrophil apoptosis
Secrete CCL2, CCL3, CCL12
Increase monocytes migration
Maintains the M1 phenotype

Question 4

Where can MSCs be isolated from?

Answer 4

Tissues - bone marrow, synovial membrane, adipose tissue, tendons, skeletal muscle

Question 5

What’s important about the tissue source?

Answer 5

Need to be sourced from healthy individuals
Elderly may have reduced immunomodulatory function
Diseased patients may have reduced therapeutic capacity

Question 6

What route of administration can MSC go through?

Answer 6

Intraorgan - local
Intravascular - systemic
Inteaperitoneal