AUC (analytical ultracentrifugation) Flashcards
1
Q
What is AUC used for?
A
Help determine the quaternary structure by studying the behaviour of macromolecules in solution under the influence of a strong gravitational force
- macromolecules either sink or float
2
Q
What is centrifugation?
A
- separation achieved by exerting a force by spinning
- force exerted is governed by distance from the axis and (speed rotation)^2
3
Q
What is the fastest shape for sedimentation?
A
A sphere
4
Q
What observations can be made using AUC?
A
dynamic behaviour (sedimentation velocity) or of systems in equilibrium (sedimentation equilibrium) - provide info about size, shape, density and conformational changes in proteins and other macromolecules
5
Q
What does the sedimentation velocity experiments show?
A
Sedimentation velocity experiments aim to interpret the entire time-course of sedimentation, and report on the shape and molar mass of the dissolved macromolecules, as well as their size-distribution
6
Q
How does the sedimentation velocity show molar mass?
A
Using the Svedberg Equation
