Lecture 3 Flashcards
How long does the ovum remain fertile for?
approx 24 hours
Sperm remain alive in tract for several days
What happens when sperm come into contact with female secretions?
They become capacitated
What is fertilised progression in mares?
Only fertilised eggs pass into the uterus which allows early pregnancy recognition
What does sperm absorb from seminal fluid?
Fructose, absorbic acid and amino acids
What allows the flagellum to power movement in the sperm?
Long mitochondria that produce ATP.
What is capacitation of sperm?
Removal of a protective coat from plasma membrane. Allows penetration of the ovum
What are the effects of capacitation of sperm?
Increased rate of metabolism - flagellum beats more rapidly - sperm more rapid
glycoprotein alteration - allows sperm-egg binding
Pro-acrosin converts to the active form acrosin whoch allows digestion of zona pellucida proteins
What is the acrosomal reaction?
Capacitated sperm migrates through follicle cell layers before reaching zona pellucida - binding induces a reaction and the sperm’s acrosome discharges contents by exocytosis
What is the prevention of polyspermy?
The plasma membrane and vitellin layer becomes depolarised
Wave of calcium sweeps across the egg - activates egg
After fertilisation it hardens and separates causing membrane
What are the 7 stages of cleavage and gastrulation stages?
Zygote - 2-cell stage - 4-cell stage - 8-cell stage - morula - blastula - gastrula
What are the 6 stages of development?
Fertilisation - cleavage - gastrulation - neurulation - neural crest - organogenesis
What is gastrulation?
Blastula develops a hole and cells start to move into it forming a gastrula.
Three layered embryo
Three layers of cells
What are the three germ layers in regards to gastrula?
Ectoderm, Mesoderm and Endoderm
In mares why does the embryo move back and forth between uterine horns?
Prevent luteolysis
What hormone is released by the embryo during implantation?
Human Chorionic Gonadotropin