RNAseq Flashcards
1
Q
List the steps involved in a general RNAseq data analyses workflow.
A
Reads mapping/assembly Normalization Read count Differential expression Functional enrichment
2
Q
What is common for all preparations of RNA for seq?
A
DNase treatment and library prep.
Do this by priming for cDNA and making this, ligate adapters and PCR amplify.
3
Q
Why normalize?
A
So that seq depth/length does not impact DE analysis
4
Q
What is read count?
A
Count number of reads for each isoform
5
Q
What is functional enrichment?
A
Identify clusters of up/downregulated
