AAMC FL#3

Question 1

To lower the pH of a buffer, what do you need to do?

Answer 1

To lower the pH of a buffer, the proportion of acidic buffer component must be increased. Adding strong base, diluting with water, or adding a different basic salt will not lower the pH

Question 2

Requirements for Michaelis-mentin kinetics experimental condition.

Answer 2

Initial velocity is measured under steady state conditions.
B.Solution pH remains constant at all substrate concentrations.
C.The concentration of enzyme is lower than that of substrate.

ALSO once the reaction reaches equilibrium, measurement of Vo will be impossible and the kinetic data will look the same regardless of substrate concentration

Question 3

What is acetylation?

Answer 3

acetylation (attachment of RC=O)

Question 4

an atom must be a __________ to coordinate to calcium ions

Answer 4

An atom must be a Lewis base to coordinate to calcium ions
Oxygen is the only Lewis basic atom present in the side chains or backbones of the listed amino acids that has either a partially negative charge (in the peptide backbone or Ser side chain) or a negative charge (in the Asp side chain). The only other Lewis basic atom present is nitrogen (distractor C), which has a partially positive chain in the peptide backbone due to resonance, and is thus less likely to coordinate calcium ions.

(1) knowing that Lewis bases coordinate with cations, (2) recognizing which side chains and backbones are Lewis bases, and (3) understanding the effect of charge on the strength of Lewis base interactions with cations.

Question 5

Southern blot uses what to differentiate between mutant and wild-type alleles??

Answer 5

Southern blot uses a restriction digest to differentiate between mutant and wild-type alleles. In order for a Southern blot to be useful, the mutation should either create or eliminate a restriction site, most of which are palindromes and 4 to 6 base pairs long.