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Semester 1 - MCBG > 3. Energy and carbohydrates 1 > Flashcards

3. Energy and carbohydrates 1 Flashcards

1
Q

Define metabolism

A

Metabolism is the set of processes which derive
energy and raw materials from food stuffs and use
them to support repair, growth and activity of the
tissues of the body to sustain life

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2
Q

Describe an overview of metabolism

A
  1. Food comes in through the GI tract
  2. Broken down to smaller molecules
  3. Absorbed into the blood where they are transported to tissues that require them
  4. In the tissues either used directly to release energy within them or interconverted into synthetic pathways to make larger molecules perhaps for storage, eg to make other proteins from the proteins that were digested.
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3
Q

What are the two metabolic pathways?

A
  1. Catabolic pathways

2. Anabolic pathways

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4
Q

Describe catabolic pathways

A

1.Break down larger molecules into smaller ones
(intermediary metabolites)
2.Release large amounts of free energy
3. Oxidative – release H atoms –‘ reducing power’

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5
Q

Describe anabolic pathways

A
  1. Synthesis larger important cellular components fro intermediary metabolites
  2. Use energy released from catabolism (ATP)
  3. Reductive(ie use H released in catabolism)
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6
Q

Do all metabolic pathways occur in all cells?

A

No

  1. Some metabolic pathways occur in all cells
  2. Others are restricted to some cell types
  3. Some may be further restricted t compartments within cells
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7
Q

In catabolic metabolism, what are the fuel molecules metabolised to supply?

A
  1. Building block material
  2. Organic precursors
  3. Biosynthetic reducing power
  4. Energy for cell function
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8
Q

Give 3 examples of building block material

A
  1. Sugars
  2. Amino acids
  3. Fatty acids
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9
Q

What are the building block materials produced in catabolic metabolism used for?

A
  1. Dynamic state of cell components (turnover)
  2. Cell growth and division
  3. Repair
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10
Q

Give an example of an organic precursor?

A

Acetyl CoA

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11
Q

Give two examples of biosynthetic reducing power

A
  1. NADH

2. NADPH

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12
Q

Define energy

A

Capacity to do work

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13
Q

What sort of work is energy needed for?

A
  1. Biosynthetic work (anabolism) - synthesis of cellular components
  2. Transport work - membranes
  3. Specialised functions
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14
Q

Give two examples of transport work

A
  1. maintenance of ion gradients (Na+,K)

2. Nutrient uptake

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15
Q

Give 3 samples of specialised functions

A
  1. mechanical work- muscle contraction
  2. Electrical work - nervous impulse contraction
  3. Osmotic work - kidney
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16
Q

What is the standard international unit for energy content for food?

A

Joule

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17
Q

What does a calorie generally mean?

A

1 kcal

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18
Q

What is basal metabolic rate?

A

Energy required by an awake individual during physical, digestive and emotional rest at 18oC
Sum of energy used by all of the tissues in the body

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19
Q

When energy intake (FOOD) > energy required, then what is the excess energy used for?

A
  1. Growth - synthesis of new tissue

2. Stored - production of adipose tissue

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20
Q

Which form f energy is predominantly used to drive energy requiring activities?

A

Chemical bond

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21
Q

Define exergonic

A

A chemical reaction which releases energy

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22
Q

Define Endergonic

A

Chemical reactions which require energy

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23
Q

Which reactions occur spontaneously?

A

Exergonic reactions

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24
Q

In which reactions are deltaG negative?

A

Exergonic

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25
Q

In which reactions are deltaG positive ?

A

Endergonic

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26
Q

Define oxidation

A

Removal of electrons or removal of H atoms

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27
Q

Define reduction

A

Gain of electrons or H atoms

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28
Q

When fuel molecules are oxidised, the electrons and protons are transferred to what?

A

Carrier molecules eg NAD, NADP

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29
Q

Carrier molecules act as carriers of reducing power for which processes?

A
  1. ATP production(NADH)

2. Biosynthesis(NADPH)

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30
Q

How many H atoms are added to the carrier molecules?

A

2 and 1 dissociates into solution

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31
Q

How can energy released as reducing equivalents be used to drive energy requiring activities?

A

1.Directly, e.g. use of NADPH in biosynthesis 2.Indirectly, e.g. mitochondrial system to couple NADH to the production of ATP

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32
Q

What is the energy released during exergonic reactions used to drive?

A

ATP production -Part of the free energy conserved as the chemical bond energy of the terminal phosphate group (PO
44-) of ATP

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33
Q

Can ATP be used as a store of energy

A

NO - only a carrier, not a store

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34
Q

What feature of ATP makes it very good as a carrier molecule

A

It is stable -does not break down

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35
Q

When ATP is high, which pathways are activated

A

Anabolic

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36
Q

When ATP levels are low and ADP and AMP levels are high which pathways are activated?

A

Catabolic

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37
Q

Which enzyme is used to produce ATP and AMO using 2ADP when ATP levels are really low?

A

Adenylate kinase

38
Q

Which forms is energy stored as ?

A

Glycogen, triglyceride

39
Q

Which is a high energy store that can be used immediately?

A

Creating phosphate

40
Q

Describe the process by which energy is stored as phosphocreatine including the enzyme used.

A

When energy supply exceeds demand, ATP combines with creatine in the presence of the enzyme creatine kinase to form ADP and phosphocreatine. Thus, the phosphate bond energy can be stored in phosphocreatine. When ATP levels are low, he phosphocreatine can combine with ADP to form ATP and creatine

41
Q

What is creatine kinase a marker of and why?

A

Myocardial infarction - CK is released from cardiac myocytes (cells) into blood when damaged, in myocardial infarction (heart attack).

42
Q

What does creatine and phosphocreatine spontaneously break down into?

A

Creatinine

43
Q

How can you obtain a measure of muscle mass and why?

A

Measuring levels of creatinine in urine as creatinine is only stored in skeletal muscle. Creatinine excretion per 24h is proportional to muscle mass of the individual
– Provides a measure of muscle mass

44
Q

Creatinine is produce by a —1— reaction at a —2— rate.

Cill in the gaps

A
  1. Spontaneous

2. Constant

45
Q

Creatinine concentration in urine is a marker of what?

A

Urine dilation - if creatine concentration is high suggests dehydrated. And vice versa. Can be used to estimate true urinary loss of many substances

46
Q

What is metabolism?

A

Inter conversion of biological molecules in small chemical steps

47
Q

What process is used to convert reducing power to ATP

A

Oxidative phosphorylation

48
Q

Catabolism involves the breakdown of chemicals to release?

A
  1. Reducing power(NADH,NADPH,FAD2H)

2. Energy(ATP)

49
Q

What is stage 1 on catabolism and what is its purpose?

A

Breakdown of fuel molecules to building block molecules

Purpose - to convert
nutrients to a form that can
be taken up into cells.

50
Q

Where does stage 1 of catabolism occur and where do the products end up?

A

Extracellular (GI tract) - Building block molecules
absorbed from GI tract into
circulation

51
Q

Is any energy released in stage 1?

A

No

52
Q

What bonds are broken in stage 1 of catabolism?

A

Breakage of C - N and C - O bonds (no C - C)

53
Q

What is stage 2 of catabolism?

A

Degradation of building block materials to metabolic intermediates and a small number
of organic precursors

(Glycolysis)

54
Q

Where does stage 2 occur?

A

Intracellular

cytosolic & mitochondrial

55
Q

Is energy released in stage 2?

A

Small fraction of energy released

56
Q

What bonds are broken in stage 2?

A

C - C bonds broken

57
Q

What kind of a process is stage 2?

A

Oxidative -release of ‘reducing
power’ (NADH) and some
‘energy’ (ATP)

58
Q

What can be said about the pathways of stage 2?

A

Many pathways

59
Q

What is stage 3 of catabolism?

A

Krebs cycle
- Acetyl coA oxidised to CO2

(produces precursors
for biosynthesis)

60
Q

Where does stage 3 occur?

A

Mitochondria

61
Q

Is energy released in stage 3?

A

Small fraction of energy released

62
Q

What can be said abut the stage 3 pathway?

A

A single pathway –
Tricarboxylic acid (TCA)
cycle

63
Q

What type of process is stage 3?

A

Oxidative

64
Q

What is stage 4 of catabolism?

A

Electron transport and oxidative phosphorylation

65
Q

Where does stage 4 occur?

A

Mitochondria

66
Q

Does stage 4 release energy

A
  • Energy released

* ATP synthesised

67
Q

What molecule does stage 4 require

A

Oxygen

68
Q

Which 4 cells have an absolute requirement for glucose?

A
  • Red blood cells
  • Neutrophils
  • Innermost cells of kidney medulla
  • Lens of the eye
69
Q

What are the enzymes involved in stage 1? Include where they are found and what they break down.

A 
>Amylase - saliva (starch,glycogen)
>disaccharidases - attached to brush border membrane of epithelial cells in small intestines 
• lactase (lactose)
• sucrase (sucrose)
• Pancreatic amylase (a1-4 bonds)
• isomaltase (a1-6 bonds)
70
Q

Why isn’t cellulose (b 1-4 bonds) digested in the human body?

A

Alpha and beta glycosidic bonds are different. Amylase can recognise the structure of alpha glycolic bonds but cannot recognise the beta bonds in cellulose so cannot break cellulose down

71
Q

What is lactose intolerance?

A

Lactose intolerance is when the body does not produce the enzyme lactase to break down and digest lactose. Therefore, lactose remains in the small intestines causing concentration in the small intestines to increase so water moves in casing diarrhoea

72
Q

What is primary lactase deficiency and who does it currently in?

A
  • Absence of lactase persistence allele.

* Only occurs in adults

73
Q

What is secondary lactase deficiency caused by and who does t occur in?

A
  • Caused by injury to small intestine:
  • Gastroenteritis
  • Coeliac disease
  • Crohn’s disease
  • Ulcerative colitis

• Occurs in both infants & adults
• Generally reversible
foods

74
Q

What causes congenital lactase deficiency?

A

• Extremely rare, autosomal recessive defect in
lactase gene.
Cannot digest breast milk.

75
Q

What are the symptoms of lactose intolerance?

A 
Symptoms:
• Bloating/cramps 
• Flatulence 
• Diarrhoea
• Vomiting 
• Rumbling stomach
76
Q

How are monosaccharides absorbed into the blood?

A

• Active transport (low to high concentration) into intestinal epithelial cells by
sodium‐dependent glucose transporter 1 (SGLT1) then,
• Passive transport (high to low concentration) via GLUT2 into blood supply

77
Q

How is glucose in blood transferred to cells?

A

Glucose uptake into cells from blood is via facilitated

diffusion using Glucose transporter proteins (GLUT1 - GLUT5).

78
Q

Where is glut 1 found?

A

In mostly all cells but mainly in Fetal tissues, adult erythrocytes, blood–brain barrier.

79
Q

Where is GLUT 2 found?

A

Kidney, liver, pancreatic beta cells, small intestine

80
Q

Where is GLUT 4 found?

A

Adipose tissue, striated muscle *insulin-regulated

81
Q

What are the functions of glycolysis

A 
• Oxidation of glucose 
• NADH production (2 per glucose) 
• Synthesis of ATP from ADP
(net= 2 ATP per glucose) 
• Produces C6 and C3
intermediates
82
Q

What are the 6 features of glycolysis?

A 
• Central pathway of CHO
catabolism 
• Occurs in all tissues (cytosolic) 
• Exergonic, oxidative 
• C6 -> 2C3 (No loss of CO2) 
• With one additional enzyme (LDH),
is the only pathway that can
operate anaerobically 
• Irreversible pathway
83
Q

Why are there so many steps in glycolysis?g

A
  1. Chemistry easier in small stages
  2. Efficient energy conservation
  3. Gives versatility
    • allows interconnections with other
    pathways
    • allows production of useful
    intermediates
    • allows part to be used in reverse
  4. Allows for fine control
84
Q

What happens in stage 1 of glycolysis and why is it done?

A

Phosphorylation of glucose to glucose-6-
phosphate (G-6-P). ATP —> ADP

• Makes glucose negatively charged
(anionic) 1
ADP
• Prevents passage back across the
plasma membrane
Glucose-6-P
• Increases the reactivity of glucose to
permit subsequent steps
85
Q

What happens in rage 2 of glycolysis?

A

Glucose-6-P converted to fructose-6-P

86
Q

What happens in stage 3 of glycolysis?

A

Fructose-6-P phosphorylated to fructose 1,6-bis-P.

ATP —-> ADP

87
Q

What kind of step is step 3 of glycolysis?

A

Committing step: first step that commits glucose to metabolism via glycolysis

88
Q

What happens in step 4 of glycolysis?

A

Cleavage of C6 into two C3 units (DHAP and glyceraldehyde 3-P)

89
Q

What happens in step 6 of glycolysis?

A

Small amount of reducing power captured (NADH).

Glyceraldehyde -P ——> 1,3-bis phosphoglycerate

90
Q

What happens in step 7 and 10 of glycolysis?

A

ATP synthesis
• 1,3-BPG and PEP(phosphoenolpyruvate —> pyruvate)
• Transfer Pi to ADP to give ATP
• ‘Substrate level phosphorylation’

91
Q

Which steps of glycolysis are reversible?

A

Steps 1,3 and 10 because have large negative deltaG values

92
Q

How can glycolyissi be used in diagnosing cancer?

A 
Rate of glycolysis up to 200
times greater in cancer.
Measure uptake of
2-18F-2-deoxyglucose (FDG)
(Radioactive glucose mimicking substrate )

Semester 1 - MCBG (18 decks)

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