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Biology 70% > Cellular process > Flashcards

Cellular process Flashcards

1
Q

Examples of biological process requiring energy

A
  • Mechanical work, e.g. muscle contraction
  • Synthesis of complex biomolecules from simple precursors
  • Generation of light (fireflies) and electricity (eels)
  • Active transport/ Neurotransmission
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2
Q

What is Heterotrophy?

A

Getting energy and volume from other organsims

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3
Q

What is chemotrophs?

A

Getting energy from chemical redox reaction

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4
Q

What is autotrophy?

A

getting energy from sunlight and volume from CO2 in the atmosphere

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5
Q

Glucose is break down into _________ by _________

A

pyruvate, glycolysis

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6
Q

Pyruvate can break down into ____?

A
  • Aerobic metabolism > Acetyl CoA (& CO2 & NAD+)

- Anaerobic metabolism > Ethanol (& CO2 & NAD+) or Lactrate (no CO2 but NAD+)

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7
Q

What is the Net production of ATP of glycolysis ?

Further ATP id produced by __________

A

2 ATP

Oxidative phosphorylation

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8
Q

What is ATP ?

A

Adenosine triphosphate. The energy currency of cell

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9
Q

Fats will be break down into ?

A

fatty acids and glycerol

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10
Q

Polysaccharides will be break down into ?

A

Glucose and other sugar

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11
Q

Protein will be break down into ?

A

Amino acids

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12
Q

Where does the critic acid cycle take place?

A

in the mitochondria

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13
Q

What is NADH?

A

A carrier of high energy e-

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14
Q

Pyruvate is combined with __________ to form Acetyl CoA, with release of ________ and production of _________

A

coenzyme A (CoA)
CO2
NADH

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15
Q

Reaction within cells is important for ____________

A
  • Synthesis of percursors

- Provision of energy

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16
Q

In metabolism, hundreds of ______________ occur

A

enzyme- catalysed reactions

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17
Q

ATP + H2o –> __________

A

ADP + Pi + energy

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18
Q

For ATP hydrolysis, AG = ?

A
  • 7.3 kcal mol-1
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19
Q

What is released when ATP is broken down?

So ATP is broken down in ________________

A

Large amount of energy

carefully controlled process and catalysed by enzyme

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20
Q

________ is needed to synthesis ATP efficiently so ___________ L of air needed to be breathe everyday

A

O2, 10,000 L

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21
Q

Turnover of ATP is high/ low?

In a sprinter, average lifespan of 1 molecule of ATP is _____

A

high

1.5 sec

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22
Q

A human hydrolyses about _____kg of ATP in 24 hours, if a person weight 70kg. And the total body ATP for is?

A

55kg > 50g

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23
Q

Different type of ATP production

A
  • Substrate level phosphorylation

- Oxidative phosphorylation

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24
Q

IF the formation of ATP is directly from metabolism pathway, it’s called _________

A

Substrate Level Phosphorylation

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25
Q

What is oxidative phosphroylation?

A

Process of ATP formation in the electron transport chain

90% of ATP in animal is formed by this method

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26
Q

What is acetaldehyde?

A

it is broken down from pyruvate and needed to be broken down again to enthanol

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27
Q

During glycolysis, NAD+ is converted to ________,

A

NADH

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28
Q

Glycolysis can’t continue if ______ decreased

A

NAD+

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29
Q

In aerobic metabolism, electrons on ________ is transferred to _________ via________________, to produce _____________

A

electrons on NADH transferred to O2 by electron transport chain to produce H2O, ATP, and NAD+

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30
Q

In anareobic metabolism, electrons on __________ transferred to _________ to form ___________

A

electrons on NADH transferred to pyruvate to form ethanol / lactate and NAD+

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31
Q

During extreme muscular activity, the oxygen delivered to the muscle is _______than the oxygen requirement for ______________

A

less than , NADH oxidation

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32
Q

What is lactate dehydrogenase?

A

Enzyme that convert pyruvate > (NADH + H+ -> NAD+) > Lactate

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33
Q

Oxygen debt is repaid in ___ mins by ____________

A

30 mins, by converting lactic acid to glucose in liver

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34
Q

Describe how body response to lactic acid production during anareobic activity

A
  • Lactic acid dissociate to lactate & H+
  • The pH decrease as H+ increased
  • Muscle pain and fail to contract
  • Activity decreases
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35
Q

Describe how blood doping aid the anareobic exercise performance

A

haemoglobin has many ionisable amino acids which may act as a buffer in skeletal muscle, which can resist the decrease in pH cause by lactic acid production

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36
Q

Vertebrates are mainly aerobic organisms T/F

A

True

37
Q

Smaller vertebrates rarely rely on_________

Larger vertebrates are often ____ moving and rarely undertake _____________activity

A

anaerobic metabolism. e.g. migrating birds fly large distances without producing an oxygen debt.

slow moving, rarely undertake intense musclar activity. e.g. elephants and aligators.

38
Q

In aerobic conditions yeast break glucose down to ___________
Yeast cells produce _________during ____________ metabolism.

A

carbon dioxide and water

ethanol , anaerobic

39
Q

Ethanol is toxic to humans but produces feelings of

A

euphoria

40
Q

Humans produce______ during anaerobic metabolism – not ___________

A

lactic acid ,

not ethanol !!!

41
Q

Acetyl CoA converted into_____________________

A

2 CO2, 8 high energy electrons and GTP

42
Q

High energy electrons released at the citric acid cycle are transferred to ________________

A

NADH and FADH2

43
Q

Describe citric acid cycle

A

C2 added to the C4 in the citric acid cycle  C6 and produce CO2 & NADH  become C5 and produce CO2 & NADH again  become C4 and give out GTP, next give out FADH2 and then NADH. The remain C4 start the cycle again

The released electrons are then transferred to oxygen in oxidative phosphorylation to produce ATP

44
Q

Describe the oxidative phosphorylation

A

The 8 e- from citric acid cycle are transferred in an electron transport chain down a series of carrier. The energy is released and used to pump the H+ ions out of the mitochondria matrix into space between the inner and outer mitochondria membrane to create a concentration gradient. The gradient is then used by the ATP synthase to produce ATP. ATP synthase is located in the mitochondria membrane and as H+ ions pass back down their concentration gradient through the narrow channel in the enzyme the energy of their flow is used to drive the production of ATP. Ultimately the electrons and H+ions are transferred to oxygen to produce water in overall reaction

45
Q

Overall a molecule of glucose generates how many molecules of ATP?

A

~ 30 molecules

46
Q

What are the major electron carriers in the oxidation of fuel molecules

A

NADH & FADH2

47
Q

In aerobic organisms how many ATP is generate from glucose oxidation.

A

26 (out of the overall 30 ATP)

48
Q

Name 2 metabolism disorders

A
  • Mercury and Arsenic toxicity

- Berberi - thiamine (vit B1) deficiency

49
Q

What is Beriberi?

A

thiamine (Vit B1) deficiency

  • Neurologic and cardiac symptoms – limb pain, muscle weakness, enlarged heart and reduced cardiac output.
  • Thiamine important in pyruvate and a -ketoglutarate dehydrogenase. These are important enzymes in the citric acid cycle.
  • Neurological symptoms as neurological tissue particularly reliant on glucose metabolism and therefore pyruvate dehydrogenase.
50
Q

What is mercury and Arsenic toxicity?

A
  • Mercury and arsenic inactive pyruvate dehydrogenase.
  • Symptoms similar to beriberi.
  • Neurological symptoms
  • The phrase “mad as a hatter” refers to hatters who were poisoned by mercury used in making hats.
51
Q

Oxidative phosphorylation is involved of _____________

A
  • electrons transport

- synthesis ATP

52
Q

The electron transport chain consists of a series of _________

A
  • oxidation/reduction (redox) couples
  • 3 protein complex
  • 2 mobile electrons carriers
53
Q

redox couples interact to carry out the overall reaction:___________
Energy is released at each redox reaction T/F

A

NADH + H+ 1/2 O2 -> NAD+ + H2O

True

54
Q

What is Cyanide?

A

A chemical compound that blocks electron flow through cytochrome oxidase by reacting with the ferric form of a heme in the complex.

55
Q

What is used to treat the cyanide block?

A

Nitrite has been used in treating cyanide poisoning as it oxidises ferro hemoglobin to ferri hemoglobin which can then complete with the cytochrome oxidase for cyanide.

56
Q

Describe the electron transport chain

A

1st: (NADH) 2 e- > NADH-Q reductase > Q & energy
2nd: FADH2 & Q > Q-Cytochrome C oxidoreductase > Energy & Cytochrome C
3rd: Cytochrome C & Cytochrome C oxidase > energy & 2H+ + 2e- + 1/2 O2 H2O

57
Q

How is energy released converted to ATP in the oxidative phosphroylation?

A

The chemiosmotic hypothesis – Electron transport and ATP synthesis coupled by a proton gradient

58
Q

Where is the ATP synthase?

A

in the mitochondria membrane

59
Q

Can the proton gradient be measured?

A

Yes

60
Q

Evidence to support the chemiosmotic hypothesis

A
  • A proton gradient can be measured
  • ATP synthesised if gradient created
  • Vesicles with ATP synthase and bacteriorhodopsin synthesise ATP when illuminated.
  • Closed compartment and intact membrane required
  • Ionophores uncouple oxidation and phosphorylation.
61
Q

During the oxidative phosphorylation, ATP is generated by:_________

A

Electrons flow from NADH or FADH2 to oxygen via a series of redox couples in the electron transport chain.

62
Q

Energy released by electron flow is converted to ATP by __________

A
  • Protons (H+) are pumped out of the matrix to generate a proton motive force.
  • ATP is generated by ATP synthase when protons flow back into the matrix.
63
Q

NADH yields how many ATP?

A

~ 2.5 ATP

64
Q

FADH2 yields how many ATP?

A

~ 1.5 ATP

65
Q

How many ATP is yield at each stage?

A

Glycolysis = 2 ATP
Citric aid cycle = 2 ATP
Oxidative phosphorylation = 26 ATP
Total = 30 ATP

66
Q

what is Photosynthesis?

A

organisms use energy from sunlight and CO2 from atmosphere to produce glucose

67
Q

Photosynthesis takes place in

A

Chloroplast

68
Q

Chloroplasts are _________ organelles in _____ cells.

A

subcellular , plant

69
Q

Chloroplast structure have________

A

inner membrane/ outer membrane / stroma / stroma lamellae/ thylakoid membrane / intermembrane space/ thylakoid space

70
Q

Plants are usually green because _____

A

Chlorophyll absorbs red and blue light and reflects green light.

71
Q

Chlorophyll is a haem like structure with a ___________ ion at the centre of the structure.

A

magnesium

72
Q

Other __________ absorb light not absorbed by chlorophyll.

The actual colour of the leaf is determined by the_______________

A

photosynthetic pigments

relative concentration of the pigments.

73
Q

Complex photosystems exist to _________

A

to channel light energy to the reaction center.

74
Q

Which molecules absorbed light, then transfer between other, until it reacts the reaction centre?

A
  • Antenna Chloroplasts
  • Carotenoids
  • Other Accessory pigments
75
Q

The role of chloroplast reaction centre

A

Photochemical reaction occurs to convert the energy of a photon into a separation of charge, initiating electron flow

76
Q

How the absorption of light energy affects the electron

A

leads to the excitation of an electron from its ground state to a higher energy level.

77
Q

Photosystems generate _________, which _______drives ATP synthesis.

A

NADPH and a proton gradient.

The proton gradient

78
Q

What is produced in photosynthesis ?

A

NADPH

79
Q

in chloroplasts, the energy harvested and ATP synthesised is used to drive synthesis of ______

A

glucose

80
Q

The role of atmospheric carbon dioxide in global warming

A

Many scientist believe that increase atmospheric carbon dioxide concentration is causing an increase in the temperature of the planet.

81
Q

example of Greenhouse gasses

A
  • Carbon dioxide (mainly produced by oxidation of fossil fuels
  • Methane (Produced by landfill and animals) – methane is a very potent greenhouse gas.
82
Q

Measurement of atmospheric carbon dioxide over time

A
  • Measure concentration of carbon dioxide in arctic and Antarctic ice caps
  • Ice has been stored in the ice icecaps for many years.
  • Collect ice from many years ago and date using carbon dating.
83
Q

Describe vertical sampling of the atomosphere

A
  • Global measurements for baseline understanding of CO2 but more detailed information required to understand regional impacts.
  • Vertical sampling network being developed over North America to determine distribution of CO2 throughout the height of the atmosphere.
84
Q

Relationship between CO2 and temperature

A
  • During last ice age (15,000 to 30,000) years ago carbon dioxide levels very low
    Warm interval 5000 years ago higher than today.
  • Long-term record suggests high CO2 associated with high temperature low CO2 associated with low temperature.
85
Q

Modern causes of increased CO2

A
  • Oxidation of fossil fuel

- Carbon released into atmosphere as carbon dioxide.

86
Q

Interventions to reduce CO2 emissions

A
  • Wind power

- Bio fuel

87
Q

Arguments against climate change.

A
  • The Earth’s climate is always changing and this is nothing to do with humans.
  • CO2 only makes up a small part of the atmosphere and so cannot be responsible for global warming.
  • Rises in the levels of CO2 in the atmosphere are the result of increased temps, not the other way round.
  • Observations of temp taken by weather balloons and satellites do not support the theory of global warming.
  • Computer models which predict the future climate are unreliable and based on a series of assumptions.
  • It’s all to do with the Sun - for example, there is a strong link between increased temp on Earth with the number of sunspots on the Sun.
  • The climate is actually affected by cosmic rays.
  • The scale of the negative effects of climate change is often overstated and there is no need for urgent action.
88
Q

About bio fuel

A
  • Burn biomass
  • Carbon still released as CO2 but this carbon recently trapped from atmosphere.
  • Completion between biofuel crop production and food crop production.
89
Q

About wind power

A
  • From Oct 2008, the UK has overtaken Denmark to become the largest producer of off-shore wind energy
  • The UK has 40% of EU’s natural wind resource
  • Offshore wind farms now have the potential to power the equivalent of around 300,000 UK homes.
  • UK now gets 3 gigawatts of electricity from wind power (enough to power 1.5 million homes)
  • Off shore wind account for 20% of that capacity
  • Wind farms are often contentious developments and gaining planning
    permission can be a problem due to public opposition.

Biology 70% (9 decks)

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