Chromatography

Question 1

What can chromatography be used for?

Answer 1

To separate and identify components of a mixture

Question 2

What are three types of chromatography? (mentioned on spec)

Answer 2

Thin-layer chromatography (TLC)
Column chromatography (CC)
Gas liquid chromatography (GLC)

Question 3

How do you calculate Rf values? (chromatography)

Answer 3

Rf = distance moved by component / distance moved by solvent

Question 4

What does separation depend on? (chromatography)

Answer 4

The balance between solubility in the moving phase and retention by the stationary phase

Question 5

How does thin layer chromatography work?

Answer 5

A plate is coated with a powdered solid and a solvent moves up the plate

Question 6

How does gas liquid chromatography work?

Answer 6

A column is packed with a solid or with a solid coated by a liquid, and a gas is passed through the column under pressure at high temperature

Question 7

What is gas liquid chromatography commonly used for?

Answer 7

To separate mixtures of volatile liquids

Question 8

What is the retention time of a substance? (chromatography)

Answer 8

The time taken for a particular compound to travel through the column to the detector

Question 9

How does column chromatography work?

Answer 9

A column is packed with a solid and a solvent moves down the column