19 + 20: Cytology Flashcards

1
Q

What gram stain are most cocci?

A

+ve

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2
Q

Are staphs and streps found in clusters or chains?

A

Staph in clusters, strep in chains

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3
Q

What gram stain are most small rods?

A

-ve

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4
Q

Which bacteria look like filamentous rods?

A

Actinomyces or Norcardia

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5
Q

Are most large rods pathogenic or non-pathogenic?

A

Non-pathogenic

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6
Q

How do you tell the difference between infection and contamination?

A

If infection, the bacteria is within the neutrophils and the neutrophils are degenerate. If contamination, the bacteria are extracellular, the population is mixed and there is no inflammatory response

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7
Q

What % of cells a mononuclear in chronic inflammation?

A

40%

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8
Q

What do a neoplastic MGN look like?

A

Variable nuclei

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9
Q

What does an activated macrophage look like?

A

Vacuoles in the cytoplasm

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10
Q

What does crypto in cats look like on cytology?

A

Signs of chronic inflamation and round crypto cells with a thick capsule

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11
Q

Which cells form the thick capsule of a granuloma?

A

FIbrocytes

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12
Q

What can the fibrocytes of a granuloma be confused with?

A

Fibrosarcoma

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13
Q

How do you tell the difference between a granuloma and a fibrosarcoma?

A

Fibrocytes in both, but other inflammatory cells in a granuloma

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14
Q

Which kind of diagnosis is hard without histo?

A

Skin disease - need architecture

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15
Q

What are two conditions where you can see eosinophils?

A

Insect bite, granuloma

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16
Q

What are the three cell types neoplasias can be?

A

Round cell, spindle cell, epithelial

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17
Q

How can you tell an FNA of a lipoma vs liposarcoma apart?

A

Both have vacuolated cytoplasm and nucleus at side. But in liposarcoma the cells can be binucleate.

18
Q

What do inclusion cysts contain?

A

Keratin/cholesterol

19
Q

Which cells can you see in an inclusion cyst?

A

Keratinised

20
Q

What do basal cell tumours look like?

A

Benign and uniform

21
Q

What can you see on histo in SCC?

A

Keratin pearls

22
Q

In which species do you see “tadpole cells” in SCC?

A

Horse, dog, not cats

23
Q

What are the cells like in SCC?

A

Large nuclei, can be binucleate, keratinocytes retain nucleus and don’t degenerate like they should, anisocytosis, neutrophils

24
Q

What features do you see in histiocytoma?

A

Round cells, binucleate, epidermis goes down into dermis in long pegs

25
Q

What features do you see in malignant histiocytic sarcoma?

A

Anisocytosis, karyocytosis, binucleate, mitotic figures

26
Q

What % of lymphomas have hyperCa?

A

50%

27
Q

Which kind of lymphomas will metastasize if not removed fast?

A

T cell

28
Q

What do plasma cells look like?

A

Bluish cytoplasm, eccentric nucleus

29
Q

Are plasma cell tumours usually benign or malignant?

A

Benign

30
Q

What do malignant plasma cell tumours look like?

A

Lots of globulin, binucleate, mitotic, “flame cells”

31
Q

What do you see on electrophoresis in a malignant plasma cell tumour?

A

Increased total protein with monoclonal peak on electrophoresis

32
Q

What do low grade melanocytes look like?

A

Black granules

33
Q

What do high grade melanocytes look like?

A

Prominent nucleoli, occasionally melanin granules

34
Q

How do you identify an amelanotic melanoma?

A

Stain

35
Q

Where are amelanotic melanomas found?

A

Oral and interdigital

36
Q

What do mast cell tumours look like?

A

Lots of granules (esp higher grade), sometimes no granules, anisocytosis, anisokaryosis

37
Q

What do mesenchymal cells look like?

A

Spindle shaped, poorly defined borders, oval nucleus, small round nucleolus

38
Q

What is the most likely diagnosis for a mesenchymal cell tumour?

A

Fibrosis or benign fibroma

39
Q

What are some features of a malignant mesenchymal cell tumour?

A

Anisocytosis, anisokaryosis, prominent nucleoli, still spindle shaped,

40
Q

What are the features of a perinanal gland adenoma?

A

Central nucleus, lots of cytoplasm, look like hepatocytes

41
Q

What are the features of a malignant adenocarcinoma?

A

Lots cytoplasm with vacuoles, multinucleation, remodelling, prominent nucleoli