Micro Lab Flashcards
what must you clean your work station with before you start work and before you leave the lab?
2% distell
where must Petri dishes with microbial growth be placed?
biohazard bins
why should Petri dish cultures always be marked on the base?
tops may become separated from base
why should you write as close to the edge of base as possible?
avoid obscuring colonies on growth medium
why must Petri dishes be incubated upside down?
prevent condensation falling on cultures and spreading them around dish
what goes in a general waste bin?
- uncontaminated waste
- hand drying tissue
- wrappers from sterile items
what goes in a biological waste bin?
- culture plates
- biological contaminated plastic tube
- spreaders
- cuvettes
- gloves
- tissues
what goes in a pot with disinfectant?
- biological contaminated plastic pastuer pipettes
- disposable spreaders
- disposable loops
what goes in a dispose jar?
pipette tips
what goes on the top shelf of a biohazard trolley?
items which need autoclaving/decontaminating
e.g. culture, flasks, tubes, universals
what goes on the bottom shelf of a biohazard trolley?
storage material
e.g. pipette tip boxes, tube tubs
what does TSA stand for?
tryptone soya agar
how big will the zone diameter be (mm) for the organism to be sensitive to antibiotic?
more than equal to 20
how big will the zone diameter be (mm) for the organism to have moderate sensitivity to antibiotic?
10-19
how big will the zone diameter be (mm) for the organism to be insensitive/resistant to the antibiotic?
less than or equal to 9
