chromatography Flashcards

1
Q

what are the 4 types of of chromatography

A

paper
column
thin layer
gas

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2
Q

what is chromatography

A

a family of separation techniques
which depends on the principle a mixture can be separated if dissolved in a solvent or carried by a gas
and the resulting solution moves over a solid

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3
Q

what is the mobile phase

A

where the molecules can move this is always a liquid or gas

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4
Q

what is the stationary phase

A

where the molecules can’t move

this must be on a solid or a liquid on solid support

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5
Q

how are substances separated in chromatography

A

substances are separated according to reactive affinity to stationary and mobile phase

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6
Q

what is the stationary and mobile phase in paper chromatography

A

stationary phase absorbent paper

mobile phase solvent

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7
Q

what is the stationary and mobile phase in column chromatography

A

stationary phase powder or a resin

mobile phase solvent

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8
Q

what is the stationary phase and mobile phase in thin layer chromatography

A

stationary phase powder(SiO2 or Al2O3)

mobile phase solvent

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9
Q

what is the stationary phase and mobile phase in gas chromatography

A

stationary phase- powder coated with oil packed into a long capillary tube
mobile phase- unreactive gas nitrogen or helium

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