Processes and techniques Flashcards
1
Q
What are the four steps of cell diff
A
Spe = restriction of potency deter = committed diff = expr of certain genes maturation = higher degree of diff
2
Q
What is growth ? What does it rely on ?
A
Increase in size or mass
Relies on prolif, enlargement, accretion
3
Q
2 theories of dev bw vert
A
Funnel = early stage similar Hourgalss = middle
4
Q
What study first ?
A
mRNA
5
Q
How do you study mRNA
A
In situ
Reporter line
High throughput analyses (microarrays)
6
Q
How study Prot
A
Western blot
Immunodetection (histichem/fluo)
Fusion protein construct
7
Q
Regulation
A
Tissue manip
8
Q
What tissues and organs derive
A
Fate mapping
