Agar types Flashcards

1
Q

What does a positive coagulase test look like?

A

Golden coloured colonies

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2
Q

What does a negative coagulase test look like?

A

Silver/white coloured colonies

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3
Q

What does the MacConkey agar test

A

Whether the organism is lactose fermenting or not

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4
Q

What does the CLED agar test

A

Whether the organism is lactose fermenting or not

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5
Q

What does the XLD agar test

A

Whether the organism is lactose fermenting or not

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6
Q

Name 5 sterile sites of the body

A
Blood
CSF
Pleural fluid
Peritoneal cavity
Joints
Urinary tract
Lower respiratory tract
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7
Q

Name 4 sites of the body that are colonised with bacteria

A
Skin
Large intestine
Mouth
Vagina
Urethra
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8
Q

What is chocolate agar?

A

Blood agar that has been heated to 80o to release nutrients

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9
Q

What does a negative MacConkey test look like and what does this mean?

A

White/colourless colonies showing non-lactose fermenting

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10
Q

What does a positive MacConkey test look like and what does this mean?

A

Red colonies showing lactose fermenting

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11
Q

Why is PCR and other NAATs used to test for viruses?

A

As viruses cannot be seen or grown easily

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12
Q

What does NAAT stand for?

A

Nucleic acid amplification test

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13
Q

Name 5 important viral infections

A
Herpes
Enteroviruses (e.g. rhinovirus)
Hepatitides (A, B, C, Delta, E)
Influenza
Measles
Mumps
Rubella
Rotavirus/norovirus
HIV
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14
Q

HIV tests can look for any of 3 markers in the blood - what are these?

A

Antibody
Antigen p24
HIV RNA

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15
Q

If an XLD plate shows red colonies WITH black centres what organism is likely to be present?

A

Salmonella

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16
Q

If an XLD plate shows red colonies WITHOUT black centres what organism is likely to be present

A

Shigella

17
Q

If an XLD plate shows yellow/orange colonies what organism is likely to be present

A

Coliforms