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Histopathology Lab > Clearing > Flashcards

Clearing Flashcards

1
Q

Other term for clearing

A

De-alcoholization

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2
Q

Process of removing alcohol or dehydrating agent from tissue and replace it with a substance that will dissolve the wax

A

Clearing

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3
Q

T/F

All clearing Agents give a transparent appearance

A

False

-chloroform can’t

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4
Q

clearing agents will make tissues transparent due to their?

A

High index refractivity

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5
Q

Clearing agents used in FSB

A

Glycerin and gum syrup

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6
Q

T/F

Clearing agents should be miscible with alcohol and paraffin

A

True

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7
Q

T/F

Most clearing agents are flammable

A

True

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8
Q

Mnemonics for characteristics of a good clearing agent

A

MM-PEMD

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9
Q

Factors affecting the rate of penetration of CA

A

Boiling point

Viscosity

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10
Q

Prolonged exposure to CA will cause

A

Brittleness (pag clear gani murag glass, DALI RA MABUAK)

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11
Q

Commonly used CA

A

Xylene

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12
Q

T/F

Xylene is not recommended for brain and CNS Tissues

A

True

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13
Q

CA recommended for urgent biopsies

A

Benzene

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14
Q

Carcinogenic CA

A

Benzene

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15
Q

CA that can be used in tissue blocks even up to 1cm in thickness

A

Chloroform

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16
Q

CA that don’t become translucent

A

Chloroform

17
Q

Recommended for tough tissues

A

Chloroform

18
Q

CA that can cause liver damage

A

Chloroform

19
Q

Tissue stem to float in this CA

A

Chloroform

-wrap with gauze to facilitate sinking

20
Q

Recommended for brain and CNS tissues, cytologic studies, smooth muscle and skin

A

Cedarwood oil

21
Q

Cedarwood oil can clear tissues in how many days?

A

Paraffin- 2-3 days

Celloidin- 5-6 days

22
Q

This chemical hasten the removal of cedarwood oil

A

Benzene

23
Q

Becomes milky upon storage

A

Cedarwood oil

24
Q

Tissue initially float in this chemical

A

Cedarwood

-superimpose absolute alcohol as remedy

25
Q

How to dissolve crystal formed in cedarwood oil?

A

Heated to 200*C

26
Q

Can clear embryos, insects, very delicate specimens

A

Aniline oil

27
Q

CA that is Very similar to chloroform but is relatively cheaper

A

Carbon tatrachloride

28
Q

CA used in double embedding

A

Methyl benzoate

And methyl salicylate

29
Q

T/F

CA can remove substantial fat from tissue

A

True

30
Q

What to do after clearing?

A

Dry first

Histopathology Lab (6 decks)

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