Intro to Microscopy

Question 1

visual enlargement of an object

Answer 1

magnification

Question 2

refers to the extent to which the detail of a magnified object is maintained/ clarity of magnified object

Answer 2

resolution

Question 3

the object will appear dark against a bright background through a condenser that allows light to pass through in parallel waves

Answer 3

bright field microscope

Question 4

the object will appear bright with a dark background because of a special sub-stage condenser that causes light waves to cross rather than pass through the specimen

Answer 4

dark field microscope

Question 5

has a special exciter filter which allows short wavelength blue light to pass and cross the specimen

Answer 5

fluorescent microscope

Question 6

allows the detection of objects exhibits birefringence or refraction of light into two slightly different positions

Answer 6

polarizing microscope

Question 7

slows down the speed of light by half a wavelength, retardation of light rays diffused by the object resulting in the halo effect allowing observation of unstained preparations

Answer 7

phase contrast microscopes

Question 8

three dimensional and layer by layer image of the specimen that appears bright against dark background

Answer 8

interference contrast microscope

Question 9

utilizes electron beams instead of light rays and magnetic fields instead of lenses allowing user to view extremely small particles such as viruses

Answer 9

electron microscope

Question 10

type of electron microscope which allows the user to view the external microstructures of the particulate matter

Answer 10

scanning electron microscope

Question 11

type of electron microscope which allows the user to view the internal microstructures of the particulate matter

Answer 11

transmission electron microscope

Question 12

generates the initial magnification of the image

Answer 12

objectives

Question 13

scanning objective (red) power

Answer 13

4x

Question 14

LPO (yellow) power

Answer 14

10x

Question 15

HPO (blue) power

Answer 15

40x

Question 16

Oil Immersion power

Answer 16

100x

Question 17

elevates or lowers the stage to roughly focus the specimen

Answer 17

coarse adjustment knob

Question 18

elevates or lowers the stage in smaller increments or premeasured distances to refine focus after shifting to higher objectives

Answer 18

fine adjustment knob

Question 19

holds the specimen in place

Answer 19

stage and stage clip

Question 20

directs light in parallel waves through the specimen

Answer 20

condenser

Question 21

moves the specimen vertically

Answer 21

specimen holder Y-axis feed knob

Question 22

moves the specimen horizontally

Answer 22

specimen holder X-axis feed knob

Question 23

compensates for the differences in eyesight between your eyes

Answer 23

diopter adjustment ring

Question 24

allows the user to shift objectives

Answer 24

revolving nosepieces

Question 25

controls the mechanism for preventing collision between the specimen and objective

Answer 25

pre-focusing knob