Unit 1 - Production of a Post Translationally Modified Protein Essay Question Flashcards
What is the 1st step for a post-translationally modified to become a functioning protein in the cell membrane?
Mature mRNA transcript leaves the nucleus and binds to a ribosome in the cytosol where translation starts.
What happens after translation begins at the ribosome?
Transmembrane proteins carry a signal sequence which tells the ribosome to halt translation and dock with the endoplasmic reticulum, forming rough endoplasmic reticulum (RER).
What is a signal sequence?
A short sequence of amino acids that stops translation of a protein at a ribosome.
What happens after the signal sequence has impacted the protein?
Translation continues after the ribosome has docked with the endoplasmic reticulum and the protein is inserted into the membrane of the ER.
What happens following translation of the protein in the membrane of the rough endoplasmic reticulum?
Once translation has finished, the protein buds off to form a vesicle and is transported along vesicles to the Golgi where the vesicles fuse.
What happens to proteins in the Golgi Apparatus?
Proteins are transported from the cis to the trans end and post translational modification takes place such as adding carbohydrate groups.
What is the final step for a post-translationally modified to become a functioning protein in the cell membrane?
Finished proteins are bound into vesicles and transported and fused with the cell membrane, then go to their final location within the cell.
