Synthetic microbiology Flashcards

1
Q

What are the three steps to making artemisinic acid?

A
  1. Engineer farnesyl pyrophosphate pathway to overproduce FPP
  2. Induce amorphadiene synthetase gene from sweet wormwood to convert FPP to amorphadiene
  3. Cytochrome P450 converts amorphadiene to artemisinic acid
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2
Q

How is bio-butanol made in E. coli?

A

2-keto acids are intermediates

Use ketoacid decarboxylases plus yeast alcohol dehydrogenase to produce butanol

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