a 2.2 Flashcards
(56 cards)
three main ideas of cell theory
1)all living things are made up of one or more cells
2)they are the basic functional units for life
3)new cells are produced from pre-existing ones
all cells have what 3 features
- surrounded by membrane
- have genetic material
- chemical reactions catalyzed by enzymes
why are stains used in micropscophy
to visualise certain chemicals
measuring using an eyepeice
this is called graticule
it is a fixed scale on an eyepeice that must be calibrated tp stage micrometer
focusuing a microsscopes
- start on lowest lense
- start with specimin far away as possible
- use coarse knob to get it right
- use fine focusing know to sharpen
all peices of microscope
- nosepecie
- eyepeice
- objective lense
- turent
- condenser and diagghram
- lamp
- stage
image size
actual x magnification
maginification
image/actual
actual
image/magnification
1 metre x 1000
1000 mm
1 mm x 1000
1000 micrometre um
1 micrometer um x 1000
1000 nanometre
scale bar represents?
size before an image was enlarged
scale bars can be used to calculate
magnification
scale bars should represnt how much of full image
20%
steps tp using scale bar
measure in MM
convert to corect unit
insert to formula
scale bar formula
scale length = lentgh it shows x magnification
magnification = scale length/lentgh it shows
lentgh shown = scale / magnification
magnification
how many times biggger the image produced is than the actual object
resolution
the ability to distiguish between two structures that are very close to one another
light microscope resolution and megnification
0.25 um and x 500
electron microscope resolution and mag
0.002 um and x500000
electron microscopes can only observe what structures due to resolution
mitrochondria, chloroplast, nucelus
transmission electron microscope
a beam of electrons goes through a specimin
the desner the part it, absorbs more electrons
give high resolution images
alows internal structures to be observed
scanning electron microscopes
beam moves across specimin
they bounce off the surface
3d image is formed
lower resolution then TEM