AA Sequence: Chemical/Enzymatic Analysis Flashcards
DNFB/Sanger Reagent
Determine N-terminal AA producing a dinitrophenol (DNP)-AA
Determine N-terminal AA producing a dinitrophenol AA
DNFB/Sanger Reagent
Phenylisothiocyanate / Edman Reagent
Determine N-terminal AA producing a phenylthiohydantoin (PTH) - AA and cleaving this from the parent peptide
Hydrazine
determine C-terminal AA
Determine N-terminal AA producing a phenylthiohydantoin (PTH) - AA and cleaving this from the parent peptide
Phenylisothiocyanate / Edman Reagent
determine C-terminal AA
Hydrazine
Cyanogen bromide
cleaves peptide bond wherein carboxyl groups are contributed by the AA;
Methionine
cleaves peptide bond wherein carboxyl groups are contributed by the AA;
Methionine
Cyanogen bromide
Hydroxylamine HCl
cleaves peptide bonds joining asparagine and glycine
cleaves peptide bonds joining asparagine and glycine
Hydroxylamine HCl
Iodosobenzoate
cleaves at carboxyl side of tryptophan residues
cleaves at carboxyl side of tryptophan residues
Iodosobenzoate
2-nitro-5-thiocyanobenzoate
cleaves at amino side of cysteine
cleaves at amino side of cysteine
2-nitro-5-thiocyanobenzoate
Trypsin
cleaves at peptide bonds wherein carbonyl group is contributed by BASIC residues, lysine and arginine
cleaves at peptide bonds wherein carbonyl group is contributed by BASIC residues, lysine and arginine
Trypsin
Chymotrypsin
cleaves at carboxyl side of AROMATIC amino acids: tyrosine, phenylalanine, tryptophan
cleaves at carboxyl side of AROMATIC amino acids: tyrosine, phenylalanine, tryptophan
Chymotrypsin
Carboxypeptidase A
cleaves all C-terminal except proline
cleaves all C-terminal except proline
Carboxypeptidase A
Carboxypeptidase B
cleaves C-terminal amino acids if lysine and arginine only
cleaves C-terminal amino acids if lysine and arginine only
Carboxypeptidase B
Thermolysin
cleaves amino side of residues where R is contributed by leucine and valine
cleaves amino side of residues where R is contributed by leucine and valine
Thermolysin
