ABO Blood Group Flashcards
(13 cards)
What is likely to happen if you transfused an incompatible ABO type?
Hemolytic transfusion reaction
– detection of antigens on individual RBCs by a known antisera
Forward grouping
– detection of antibodies on individuals’ serum by a known reagent RBCs (A1 and B cells)
Reverse grouping
Identify the lectin.
- agglutinates A1 or A1B
Dolichos biflorus
Identify the lectin.
- agglutinates B cells
Bandeiraea simplicifolia
Identify the lectin.
- agglutinates O cells (H specificity)
Ulex europaeus
What temperature do ABO antibodies react best?
20-24℃
What temperature are complements activated?
37℃
identify sugar present
group A
N-acetylgalactosamine
identify sugar present
group B
D-galactose
identify sugar present
group O
L-fucose
predominant immunoglobulin in ABO
IgM
rare phenotype in Px that lacks H Ag
Bombay phenotype