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Accessioning (Sir Gams PPT) Flashcards

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1
Q

Anatomic Pathology Laboratory Receiving

A
  • Receiving (Request form, specimen)
  • Logging
  • Accessioning/ Numbering
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2
Q

T/F: Tissues removed from the body can be fixed within 1 hour of its removal.

A

FALSE, it should be fixed IMMEDIATELY!

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3
Q

The fixative should be __________ of the volume of the specimen

A

More than 10x

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4
Q

Process of giving an identifying marker to a
specimen

A

Accessioning/ Numbering

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5
Q

Accessioning starts in which area of the laboratory?

A

Receiving area

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6
Q

Specimens are logged based on the time it was?

A

Received

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7
Q

T/F: The middle name of the patient can be omitted from the request form.

A

FALSE, it should be included.

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8
Q

T/F: The date and time of request is the same as time of specimen removal.

A

FALSE, it is different.

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9
Q

Contents of clinical abstract

A
  • Medical history of patient
  • Chief complaint
  • Details of the patient’s case
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10
Q

T/F: The tissue can be processed even if the information is not complete since it can be followed up.

A

FALSE, NEVER process samples with INCOMPLETE information

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11
Q

T/F: If the specimen number is S-2018-3IMT-2020, the specimen was received in the year 2020.

A

FALSE, it was received in the year 2018

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12
Q

Fixative for immunofluorescence

A

Saline or liquid nitrogen

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13
Q

This is used for renal and skin biopsies

A

Immunofluorescence

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14
Q

In immunofluorescence, __________ are utilized to detect a specific location in the tissue.

A

Antibodies

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15
Q

This provides rapid diagnosis

A

Rush Frozen Section

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16
Q

T/F: The fixative for the Rush Frozen section is formalin.

A

FALSE, there are no fixatives used in this section.

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17
Q

Specimens in the Rush Frozen section uses?

A

Liquefied CO2 or Liquid nitrogen

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18
Q

Clinical application of Rush Frozen section

A

Hirschsprung disease

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19
Q

This provides a high magnification to be able to see the ultramicroscopic details of a cell.

A

Electron Microscopy

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20
Q

T/F: Specimens are rejected IMMEDIATELY if the criteria is not met.

A

FALSE. Since specimens in histopathology are difficult to replace, troubleshoot first.

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21
Q

It is grounds for specimen rejection if the tissue has already gone?

A

Putrefaction

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22
Q

Ratio of tissue to formalin

A

1:10 or 1:20

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23
Q

Container for specimens

A

Leak-proof, must fit the specimen

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24
Q

Transport time should be _________________ of collection

A

Within 1 hour

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25
Specimen Rejection criteria
- Condition of the specimen is not ideal for the test (unfixed/fixed) - Quantity of fixative not sufficient - Wrong container - Long transport time - Wrongly or incomplete filled request form - Unlabelled/Mislabeled specimen
26
Receiving: Logging and Numbering
1. Verify information on the request form 2. Verification of the specimen label 3. Enter all data in the logbook/LIS 4. Accession/Number the specimen
27
Importance of Accessioning
- Avoid mixing/switching of specimen - Track down specimen/results - Prevents mismanagement of specimen - Process the right specimen - Accurate and Timely results - Proper Management/treatment at the right time - Record keeping - Laboratory Planning
28
This is the first step in Histopathologic techniques
Accessioning/Numbering
29
Purpose of Accessioning
Identify specimen without writing the name of the patient
30
Initial assessment of the specimen
Accessioning/Numbering
31
All requisition forms must be labeled with correct information of the patient:
- Name of Patient - Age, DOB, Sex - Medical Record Number - Date and Time of Collection - Type of Specimen (anatomic site) - Location of the patient - Clinical History of the Patient - Pre/post operation diagnosis - The requesting physician's name - Physician or authorized person's signature on the request form - Name and address of physician (for referral laboratories)
32
This is required in the request form if it is intended for referral laboratories
Name and address of physician
33
Specimen container must be labelled with the correct information:
- Full name of the patient - Medical record number - Age and sex of patient - Type of specimen (Anatomic site) - Date and time of collection - Location of the patient
34
Identity of every specimen is maintained at all time using these two identifiers:
Medical Record Number and Pathology Number
35
If there are multiple specimens from the same patient, they can be labeled as:
A, B, C, etc.
36
If the specimen has different blocks, they can be labeled as:
A1, A2, A3, etc.
37
If the specimen blocks have different slides, they can be labeled as
A1-1, A1-2, A1-3, etc.
38
Slides should be labeled using a:
Permanent marker/pen
39
T/F: If H&E is the stain used, it should be indicated.
FALSE, all slides other than those stained by H&E will bear the name of the stain
40
Specimens must be labelled with how many identifiers:
Two
41
T/F: Sub-optimal specimens should be rejected right away.
FALSE, the physician should be notified first, and resolve the problem
42
T/F: If the sub-optimal specimen is accepted after the physician has resolved the problem, it is not necessary to document it.
FALSE
43
All specimens from outside the hospital must have the approval of the hospital administration and consultant on duty before they are accepted for processing. This rule is waived if the specimen is coming from a:
Government hospital
44
Requests for frozen section require how many hours of notice ahead of the operation?
24 hours
45
T/F: All In-patients for histopathologic diagnosis must have their laboratory tests entered and collected in the computer by the attending physician.
TRUE
46
The Histopathology requisition form is also known as
Form Lab 1-A
47
All specimens must be received fixed with 10% buffered formalin except:
- Frozen section - Immunofluorescence - Electron microscopy
48
Electron microscopy specimens should be fixed in:
2% glutaraldehyde solution in the cold after excision
49
Renal biopsies should have advance notice before the procedure. Call __________ before the procedure.
15 minutes
50
T/F: A medical technologist will be available on site to perform renal biopsy.
FALSE, Pathologist
51
Renal biopsy specimens are examined under which microscope?
Dissecting microscope
52
What do we look for in Renal biopsy under the dissecting microscope?
Glomeruli
53
Renal biopsy specimens are divided into 3 parts:
1. Frozen (immunofluorescence) 2. Immersed in glutaraldehyde (electron microscopy) 3. Fixed in formalin (paraffin processing)
54
T/F: Renal biopsy specimens must be sent to the lab within one hour after the renal biopsy procedure.
TRUE
55
T/F: The specimen in saline should be immediately snap frozen in liquid nitrogen for further frozen sectioning and immunofluorescence staining.
TRUE
56
Fixative for Testicular biopsies
Bouin's fluid
57
Surgical margins for breast lump
Oriented by sutures; normal tissues surrounding the lesions or lump
58
Mastectomy apical nodes
Indicated by sutures
59
T/F: For mastectomy specimens, Superior margin should be indicated by a separate suture in case of simple mastectomy (without axillary dissection).
TRUE
60
T/F: Perinodal fat should not be included.
FALSE, inclusion of perinodal fat ensures the integrity of the lymph node capsules and assess the tumor spread beyond the lymph node capsule
61
Infective specimen should be clearly marked with:
Red marker
62
T/F: The proximal or distal excised margins of bowel resection should be oriented by a suture.
TRUE
63
T/F: Skin biopsies intended for immunofluorescence are fixed with formalin.
FALSE, it should be received frozen
64
T/F: Tiny and small biopsies from endoscopies and dermatology are submitted for processing on the same day, if received before the cut-off time and is fixed.
TRUE
65
Specimens are usually kept at room temperature for at least how many weeks after the release of report?
at least 2 weeks
66
Referral slides/blocks must be accompanied by the official original pathology report with the following information:
- Patient's name - Medical record number - Hospital/ clinical pathology number - Number of slides/blocks
67
Exempted from Pathology Examination:
- Foreign bodies - Hair, finger nails, toe nails removed for cosmetic purposes - Teeth, dental appliances - Eye Lens - Calculi - Nasal septum cartilage and bone - Foreskin from circumcision
68
"Gross Description Only"
- Torn meniscus - Varicose veins - Bone fragments from non-pathologic fracture - Ear cartilage - Fetus
69
T/F: Microscopic examination should be performed whenever there is a request by the attending physician, or at the discretion of the pathologist when indicated by the clinical history or gross findings.
TRUE, irrespective of any exemptions
70
Small specimens must be sealed in
Biohazard labeled bags
71
Large specimens must be covered in
Tight, leak-prof containers

HISTOPATH (3 decks)

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