ACT 9

Question 1

Activity No. 9 Title

Answer 1

Estimation of Total Flavonoids in Methanolic Solution of Crude Plant

Question 2

are almost present in all parts of the plant including the fruits, roots, leaves and outer skin of the stem.

Answer 2

Flavonoids

Question 3

A number of medicinal plants containing flavonoids have been reported to have (5)

Answer 3

antioxidant
antibacterial
antiviral
anti-allergic
anticancer activities

Question 4

Give two objectives of the experiment.

Answer 4

1. To determine the flavonoids level in methanolic solution of crude plant extract
2. To estimate the total flavonoids present in plant samples.

Question 5

In determination of flavonoid levels, what flavonoid is used?

Answer 5

100 ppm Quercetin standard

Question 6

Volumes of quercetin standard

Answer 6

100, 200, 300, 400, 500 microL

Question 7

Chemicals used in determination of flavonoids (3)

Answer 7

1% Aluminum chloride
1 M Sodium Acetate
100 ppm Quercetin Standard

Question 8

Add into each flask ______ microL of 1% Aluminum chloride (AlCl3) and ______ microL of 1 M Sodium Acetate (NaC2H3O2 )

Answer 8

100 microL Aluminum chloride
100 microL Sodium Acetate

Question 9

Chemical used to dilute and finalize the volume of each flasks.

Answer 9

Methanol

Question 10

How many minutes you incubate your mixture at room temperature?

Answer 10

5 minutes

Question 11

In determination of flavonoid levels, measure the absorbance at _______ nm using the ______________________ after incubation.

Answer 11

454 nm
Double Beam UV-Visible Spectrophotometer

Question 12

What unknown chemicals are used in the analysis of sample?

Answer 12

MX3 capsule methanolic solution
Cucumber leaf extract methanolic solution

Question 13

In analysis of sample, what volume of alcohol extract solution is transferred to 10-ml volumetric flask?

Answer 13

200 microL

Question 14

Concentration of Quercetin (6)

Answer 14

0.0
1.0
2.0
3.0
4.0
5.0 mg/L

Question 15

Y axis in plot

Answer 15

Absorbance at 454 nm

Question 16

X axis in plot

Answer 16

Quercetin concentration