Activation of Foam Positive Proteins Flashcards

(18 cards)

1
Q

What is beer foam composed of?

A

Bubbles of gas surrounded by beer

The foam’s stability is influenced by amphiphilic substances in beer.

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2
Q

What are the two proteins identified as particularly good at stabilizing beer foam?

A
  • Protein Z
  • Lipid transfer protein one (LTP1)

These proteins help maintain the structure of foam in beer.

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3
Q

What percentage of non-dialysable protein does protein Z account for in beer?

A

10-25%

Protein Z is the first protein identified as foam positive.

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4
Q

What is the molecular weight of protein Z?

A

Around 45 kDa

This characteristic is important for its function in beer.

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5
Q

What reaction is thought to glycate protein Z?

A

Maillard reaction

Glycation involves the attachment of carbohydrates to the protein.

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6
Q

How does glycosylated protein Z compare to non-glycosylated protein Z in terms of foam promotion?

A

Glycosylated protein Z is much more effective at promoting foam

This enhancement is crucial for beer foam stability.

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7
Q

What is the role of LTP1 in barley?

A

Transfers lipids and protects barley from microbial pathogens

This function is essential for the barley’s health.

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8
Q

What happens to LTP1 when it is denatured during wort boiling?

A

It becomes more hydrophobic

This change allows it to associate with gas in foam bubbles.

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9
Q

What is the effect of boiling wort under pressure at 103°C on foam stability?

A

Results in poor foam stability

This highlights the importance of temperature control during boiling.

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10
Q

How does a 102°C boil compare to a 96°C boil in terms of foam stability?

A

102°C boil produced poorer foam stability than 96°C boil

The level of lipids in the beer influences this outcome.

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11
Q

What is the purpose of adding antifoam during boiling?

A

To minimize foam production in the kettle

This prevents the loss of compounds that support foam and reduces the risk of overflow.

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12
Q

What is the most popular antifoam used in brewing?

A

Dimethylpolysiloxane

It is added at a rate of a few mg/L before the boil.

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13
Q

What is trub formation in the brewing process?

A

Protein coagulation and flocculation

Trub formation is influenced by temperature, time, and vigour.

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14
Q

What is the mechanism behind bitterness extraction during boiling?

A

α-Acid extraction and isomerisation

This process depends on temperature, vigour, and time.

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15
Q

What processes contribute to flavour and colour development in beer?

A
  • Maillard reaction
  • Strecker degradation
  • Caramelisation
  • Hop aroma extraction

These reactions are affected by temperature, time, and pH.

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16
Q

What is the role of denaturation of enzymes during boiling?

A

Stabilisation

It also contributes to sterilisation and trub removal.

17
Q

What compounds are removed during the volatile removal process in brewing?

A
  • DMS
  • Worty/grainy aldehydes
  • Maillard products

This process is influenced by vigour, time, and temperature.

18
Q

What happens during concentration in the boiling process?

A

Evaporation of water

This process is also dependent on vigour, time, and temperature.