ADENO Flashcards
(24 cards)
serotypes that causes respiratory diseases most common in infants and children (group c viruses)
1,2,5
serotypes that causes respiratory diseases in adolescents and adults
3,4,7
serotypes that causes responsible for 10-20 % of childhood pneumonia
3,7,21
serotypes for Acute respiratory disease in military
recruits
4,7 (occasionally 3)
Pharyngoconjunctival fever
(“swimming pool conjunctivitis”)
3,7
Epidemic keratoconjunctivitis
(“shipyard eye”
8,19,37
Gastrointestinal disease:
infantile gastroenteritis
viral gastroenteritis in young children
40,41
Severe respiratory disease in
immunocompromised patients — may
progress to fatal pneumonia
1-7
Acute hemorrhagic cystitis in children,
especially boys — virus often present
in urine
11,21
Duration of adenovirus excretion (throat of adults with common cold)
1-3 days
Duration of adenovirus excretion in throat, stool, and eye, for
pharyngoconjunctival fever
3-5 days
Duration of adenovirus excretion in eye, for keratoconjunctivitis
2 weeks
Duration of adenovirus excretion urine, throat, and stool of
immunocompromised patients
2-12 months
Duration of adenovirus excretion throat and stool of children with
respiratory illnesses
2-6 weeks
routinely used for rapid diagnosis of adenovirus
infections in tissue samples or body fluids
POLYMERASE CHAIN REACTION
can detect and identify all known serotypes of
human adenoviruses
pcr
measure type-specific antigens and can be used to identify
specific serotypes
HEMAGGLUTINATION-INHIBITION AND
NEUTRALIZATION TEST
human embryonic kidney cells , HEp-2, HeLa, and KB, are
sensitive but difficult to maintain
Isolates can be identified as adenoviruses by
immunofluorescence tests using an
antihexon antibody
on infected cells
specimen for adenovirus
stool or urine or throat, conjunctival, or rectal
swab
for rapid detection of infectious adenovirus
SHELL VIAL TECHNIQUE
SHELL VIAL TECHNIQUE cultures are incubated for
1-2 days
can identify an isolate as an adenovirus and group it
VIRAL DNA BY HYBRIDIZATION OR RESTRICTION
ENDONUCLEASE DIGESTION PATTERNS
can be used for the detection of fastidious enteric
adenoviruses in fecal extracts
ELECTRON MICROSCOPY, BY ENZYME-LINKED
IMMUNOSORBENT ASSAY, OR BY LATEX
AGGLUTINATION TEST
infections in children with heart
transplants
myocardial adenovirus infections
