Advanced Molecular Techniques Flashcards

1
Q

How can DNA be analysed at the nucleotide level?

A

DNA sequencing, PCR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How can DNA be analysed at the gene level?

A

Southern hybridisation, northern hybridisation, RT-PCR, microarray, DNA fingerprinting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

How can DNA be analysed at the chromosome level?

A

Karyotyping, FISH/chromosome painting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

When do we use reverse transcriptase PCR?

A

To detect RNA expression (TAQ polymerase does not recognise RNA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is microarray?

A

A set of DNA sequences representing the entire set of genes of an organism, arranged in a grid pattern for use in genetic testing.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What can microarray tell us eg in cancer cells?

A

Which genes are switched on/off in cancer cells compared to normal cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is used as a probe in DNA fingerprinting?

A

Minisatellite - lots of repeated areas of about 30 bases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What can DNA fingerprinting show?

A

Family relationships

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is karyotyping?

A

Tests to identify and evaluate the size, shape, and number of chromosomes in a sample of body cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is FISH?

A

Technique that uses fluorescent probes that bind to only those parts of the chromosome with a high degree of sequence complementarity.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly