AGAPPE FINALS

Question 1

HDL cholesterol is linear up to _______?

Answer 1

125 mg /dl

Question 2

Method of HDL use

Answer 2

Precipitation method

Question 3

___________ are the proteins, which mainly transport lipids in the blood stream.

Answer 3

Lipoproteins

Question 4

LDL carries cholesterol to the peripheral tissues where it can be deposited and increase the risk of ____________and ___________

Answer 4

atherosclerotic heart and peripheral vascular disease

Question 5

Hence high levels of HDl are ___________

Answer 5

atherogenic

Question 6

serum HDL helps identify patients a t risk developing _________

Answer 6

coronary heart disease

Question 7

The chylomicrons, VLDL, LDL are precipitated by ________

Answer 7

phosphotungstic acid and magnesium ions

Question 8

After centrifugation high density are in the _______

Answer 8

supernatant

Question 9

HDL content of supernatant is measured by an __________

Answer 9

enzymatic method

Question 10

description of HDL cholesterol reagent

Answer 10

phosphotungstic 14 mmol
magnesium chloride 1 mmol
preservatives

Question 11

description of HDL cholesterol standard

Answer 11

HDL cholesterol standard concentration 50 mg/dl

Question 12

product code of HDL cholesterol reagent

Answer 12

4 X 25

Question 13

product of HDL cholesterol standard

Answer 13

1 X 4

Question 14

interferences of HDL

Answer 14

bilirubin - 10 mg/dl
hemoglobin - 1000 mg/dl

Question 15

materials provided in hdl cholesterol

Answer 15

hdl cholesterol standard and hdl cholesterol reagent

Question 16

materials required but not provided
(6)

Answer 16

Cholesterol reagent
Pipettes and tips
Test tubes and racks
Timer
Incubator
Analyzer

Question 17

HDL

Test of parameters
Mode of reaction
Slope of reaction
Wavelength
Wavelength
Temperature
Standard concentration
Blank
Linearity
Incubation time
Sample volume
reagent volume
cuvette

Answer 17

endpoint
increasing
505 (500-550 nm)
630 nm
37 C
125 mg/dl
Cholesterol reagent
125 mg /dl
5 minutes
50 ul
1000 ul
1 cm light path

Question 18

traditional unit
SI unit
Conversion from Tradition to SI

Answer 18

mg/dl
mmol/L
X0.026

Question 19

I. precipitation
sample for HDL
HDL reagent

Answer 19

300 uL
300 uL

Question 20

HDL
Mix well, allow to stand for 10 min at room temperature, mix again and centrifuge for 10 min, at _____________

Question 21

HDL
Mix well, allow to stand for _________at room temperature, mix again and centrifuge for __________, at _____________

Answer 21

10 min/10 min/ at 4000rpm

Question 22

HDL cholesterol determination

cholesterol reagent
HDL standard
HDL supernatant

Answer 22

1000 uL
1000 uL
1000 uL
50 uL
50 uL

Question 23

HDL cholesterol and determination
Mix well and incubate for ________ at ________ measure the absorbance of standard and sample against the reagent bottle

Answer 23

5 minutes at 37 C

Question 24

Calculation of HDL cholesterol

Answer 24

HDL cholesterol conc (mg/dl) = absorbance of sample
/ absorbance of standard X concentration of standard X 2

Question 25

3 performance of HDL

Answer 25

1. linearity 2. Precision 3. Sensitivity

Question 26

It is recommended to establish its own reference values

Answer 26

Reference range

Question 27

HDL cholesterol of men and women

Answer 27

35-55 mg/dl 45-55 mg/dl

Question 28

LDL cholesterol of men and women

Answer 28

suspicious 150 mg/dl elevated 190 mg/dl

Question 29

HDL reagent is linear up to ____________

Answer 29

125 mg/dl

Question 30

Precision HDL qualicheck norm under expected value and measured value qualicheck path under expected and measured value

Answer 30

30 +- 4.5 32 70 +- 40 68.51

Question 31

HDL lower sensitivity limit is _________

Answer 31

1.0 mg/dl

Question 32

Triglycerides methodology use

Answer 32

GPO TOPS Glucose phosphate oxidase

Question 33

Triglycerides is linear up to __________

Answer 33

1000 mL

Question 34

Contains LCF which minimizes rerun

Answer 34

Triglycerides

Question 35

__________ are simple lipids, formed in the liver by _______ and ________

Answer 35

Triglycerides glycerol and fatty acids

Question 36

Triglycerides transported by VLDL, LDL, and constitute about _____ of fat, stored as source of energy in the _____ and _________

Answer 36

95% ------ tissue and plasma

Question 37

Increased levels of triglycerides are found in _______, _________, _______, _________

Answer 37

hyperlipidemias diabetes nephrotic syndrome hypothyroidism HDNH

Question 38

Increased levels are risk factor for ___________, ________, ________, ____________

Answer 38

arteriosclerotic coronary disease peripheral vascular disease acute pancreatitis hyperlipoproteinaemia APAH

Question 39

decreased levels are found in _________ and ___________

Answer 39

malnutrition and hyperthyroidism

Question 40

GPO LPL GK

Answer 40

glycerol 3 phosphate oxidase lipoprotein lipase glycerol kinase

Question 41

Triglycerides standard product code

Answer 41

1X 4 mL

Question 42

triglycerides standard description

Answer 42

triglycerides standard concentration 200 mg/dl

Question 43

give me the 9 under kit components

Answer 43

risk and safety reagent preparation reagent storage and stability open vial stability on board calibration stability storage detoriation precaution waste management sample

Question 44

Test parameters Triglycerides Mode of reaction slope of reaction wavelength 1 wavelength 2 temperature Standard concentration linearity blank incubation time sample volume reagent volume cuvette

Answer 44

end point increasing 546 - 540-560nm 630 nm 37 C 200 mg/dl 1000 mg/dl reagent 5 minutes 10 ul 1000 ul 1 cm light path

Question 45

traditional unit SI unit Conversion from Traditional to Si

Answer 45

mg/dl mmol/L X0.0114

Question 46

Reference range in male and female triglycerides

Answer 46

Men 60-165 mg dl Women 40 - 140 mg dl

Question 47

Sensitivity in triglycerides Lower detection is ____

Answer 47

2 mg dl

Question 48

Calculation of triglycerides

Answer 48

Tri conce mg dl - absorbance of sample/ absorbance of standard X 200

Question 49

Linearity of triglycerides

Answer 49

1000 mg dl

Question 50

what is the methodology use by CHOLESTEROL

Answer 50

CHOD PAP - cholesterol oxidase phenol 4 aminoantipyrine peroxidase

Question 51

Linearity of cholesterol

Answer 51

600 mg dl

Question 52

Increased level of cholesterol are found in

Answer 52

Hypercholesterolaemia Hypothyroidism Hyperlipidaemia Uncontrolled diabetes Nephritic syndrome Cirrhosis

Question 53

It is the main lipid found in the blood, bile, brain tissues

Answer 53

Cholesterol

Question 54

Decreased level of cholesterol

Answer 54

Malabsorption, malnutrition, anaemia, hyperthyroidism, liver disease