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CCP4.1: Pharmaceutical Biochemistry, Lab > Amino Acids > Flashcards

Amino Acids Flashcards

(59 cards)

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1
Q

building blocks of proteins

A

Amino acids

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2
Q

organic molecule containing both carboxyl acid and amino functional groups

A

Amino acids

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3
Q

can be grouped as nonpolar,polar,acidic,basic

A

Amino acids

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4
Q

capable of being converted to glucose/sugar

A

Glucogenic Amino acid

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5
Q

capable of being converted to ketone

A

Ketogenic Amino acid

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6
Q

a process of changing/deviating from the native conformation

A

Denaturation

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7
Q

Denaturing agents:

A
  1. Heat
  2. Extreme pH
  3. Mercaptoethanol
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8
Q

constitute of 70% of the organic matter of a cell

A

Proteins

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9
Q

are polymers of amino acids linked by peptide bonds

A

Proteins

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10
Q

globular:

A

globule (ex.albumin)

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11
Q

fibrous:

A

large

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12
Q

structural protein;made up of 2AA

A

Collagen

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13
Q

blood vessels

A

Elastin

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14
Q

storage proteins, muscles

A

Myoglobin

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15
Q

transport proteins, carries O2 blood

A

Hemoglobin

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16
Q

hormones;controlling blood sugar

A

Insulin & Glucagon

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17
Q

antibodies

A

Immunoglobulins

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18
Q

Isolation/Sequencing of Proteins

A
  1. By polarity: salting out, precipitation
  2. By charge
  3. By size
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19
Q

technique of separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream

A

Chromatography

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20
Q

_______phase may be either a liquid or a gas

A

Mobile phase

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21
Q

________phase is either a solid or a liquid

A

Contiguous stationary phase

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22
Q

Mobile phase: Non-polar
Stationary phase: Polar

A

Normal

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23
Q

Mobile phase: Polar
Stationary phase: Non-polar

A

Reverse

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24
Q

Xanthoproteic Test: Reagent

25
Xanthoproteic Test: Positive
Yellow or Orange ppt
26
Xanthoproteic Test:Presence
Phenylalanine
27
Fohl’s Test: Reagent
PbCH3COO
28
Fohl’s Test:Positive
Black ppt (PbS)
29
Fohl’s Test:Presence
Methionine
30
Hopkin-Cole Test:Reagent
Glyoxylic acid
31
Hopkin-Cole Test:Positive
Violet ring
32
Hopkin-Cole Test:Presence
Tryptophan
33
Ninhydrin Test:Reagent
Ninhydrin rgt
34
Ninhydrin Test:Positive
Primary: Violet Secondary: Yellow
35
Ninhydrin Test:Presence
General test for free amino acids
36
Millon-Nase:Reagent
Hg
37
Millon-Nase:Positive
Pink/old Rose
38
Millon-Nasse:Presence
Phenolic rings, Tyrosine
39
Sakaguchi Test:Reagent
a-naphthol
40
Sakaguchi Test:Positive
Red/wine color
41
Sakaguchi Test:Presence
Arginine
42
protein made by the liver
Albumin
43
it keeps fluid from leaking out of blood vessels, nourishes tissues and transports hormones, vitamin, drug and other substances throughout the body
Albumin
44
Disease related to albumin deficiency
1. Severe liver disease 2. Kidney disease
45
family of proteins found in grains like wheat, rye, spelt and barley
Gluten
46
two main proteins found in gluten
Glutenin and Gliadin
47
most severe form of gluten intolerance
Celiac disease
48
autoimmune disease that involves the body from treating gluten as an invader
Celiac disease
49
Common sources of gluten:
1. Wheat 2. Spelt 3. Rye 4. Barley 5. Bread 6. Pasta 7. Cereals 8. Beer 9. Pastries
50
When wheat flour is washed this process removes the starch that when tested with I2 solution _____results give ______ solution
Positive results give violet or purple solution
51
The ________ material left is the gluten
Insoluble material
52
vital to oxygen transport and storage in vertebrates
Myoglobin and Hemoglobin
53
dominant in muscles
Myoglobin
54
dominant in blood
Hemoglobin
55
myoglobin can be isolated by using _________precipitation
ammonium sulfate precipitation
56
a common technique used to identify compounds, determine purities
Thin layer chromatography
57
also used to separate or differentiate substances according to their polarity
Thin layer chromatography
58
semi-quantitative method
Thin layer chromatography
59
special finely ground matrix (silica gel, alumina) is coated on a glass plate or a metal or plastic film as a thin layer
Stationary phase: Thin layer chromatography

CCP4.1: Pharmaceutical Biochemistry, Lab (1 decks)

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