Amino acids (Enzymology 1st partial) Flashcards

1
Q

Biomolecules

A
  1. Proteins
  2. Carbohydrates
  3. Lipids
  4. Nucleic Acids
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2
Q

Remarks on enzymes

A
  • Biomolecules that catalyze chemical reactions by 10^15 to 10^17 fold
  • Almost all enzymes are proteins
  • Molecules at the beginning of the process are called substrates.
  • Enzymes convert them into different molecules, products
  • Living systems use enzymes to accelerate and control the rates of vitally important biochemical reactions
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3
Q

What is the earliest known use of enzymes? (Ancient Egypt)

A

Use of yeast for fermentation of wheat -> Boza

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4
Q

Who made a study of the fermentation of sugar to alcohol by yeasts? (1800s)

A

Louis Pasteur

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5
Q

Who was the first to use the term enzyme to describe a process called fermentation? (Late 1800s)

A

Wilhelm Kühne

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6
Q

Word that is used to refer to chemical activity produced by living organisms… (Late 1800s)

A

Ferment

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7
Q

Word that was used to refer to nonliving substances (such as pepsin)

A

Enzyme

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8
Q

Who began to study the ability of yeasts extracts to ferment sugar? (Late 1800s to Early 1900s)

A

Eduard Buchner

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9
Q

What did Buchner find?

A

The sugar was fermented even when there were no living yeasts cells in the mixture.

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10
Q

What is the name of the enzyme that brought about the fermentation of sucrose?

A

Zyamase

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11
Q

Area of applied science related to fermentation that deals with the biochemical processes involved in fermentation, with yeasts and bacteria selection and physiology

A

Zymology

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12
Q

Who won the Nobel Prize in Chemistry in 1907? And for what?

A

Buchner for his biochemical research and his discovery of cell-free fermentation

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13
Q

Who won the Nobel Prize in Chemistry in 1929? And for what?

A

Arthur Harden and Hans von Euler-Chelpin for their investigations on the fermentation of sugar and fermentative enzymes

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14
Q

Who increase the knowledge about yeast and brewing nowadays?

A

Danis Carlsberg lab scientists

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15
Q

What is the controversy on 1900s about enzymes?

A

Many early workers noted that enzymatic activity was associated with proteins, but several scientists argued that proteins were merely carriers for the true enzymes and that proteins per se were incapable of catalysis.

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16
Q

Who showed that the enzyme urease (pure protein) and the enzyme catalase can be crystallized?

A

James B. Sumner

17
Q

Who proved that pure proteins can be enzymes?

A

Northrop and Stanley in 1930

18
Q

Who won the Nobel Prize in Chemistry in 1946? And for what?

A

1) James Batcheller Sumner for his discovery that enzymes can be crystallized
2) John Howard Northrop and Wendell Meredith Stanley for their preparation of enzymes and virus proteins in a pure form

19
Q

Who studied autocatalytic (cuts itself) properties of RNA in 1980s?

A

Cech

20
Q

Who was studying the processing of tRNA and isolated and enzyme called RNase P (only natural true ribozyme)?

A

Altman

21
Q

Who won the Nobel Prize in Chemistry 1989? And for what?

A

Sidney Altman and Thomas R. Cech for their discovery of catalytic properties of RNA

22
Q

What is the monomer or L amino acid structure?

A
  • Amino group
  • Alpha Carbon
  • Side-chain R group
  • Alpha carboxylate group
23
Q

Amino acid classification

A
  • Polarity of side chains (hydrofobicity)
  • Basicity or acidity
  • Essentials / No essentials
24
Q

Amino acid nomenclature

A
  • 3 letter codes

- 1 letter codes

25
Q

What amino acid is not chiral?

A

Glycine

26
Q

How many amino acids are created in a synthetic way?

A

80

27
Q

How many standard amino acid are they?

A

20

28
Q

How many proteinogenic amino acids are they?

A

22

29
Q

How many amino acids are produced by natural pathways?

A

900

30
Q

How many engineered amino acids have been placed into a protein?

A

118

31
Q

Basic amino acids (positive)

A
  • Lysine
  • Arginine
  • Histidine
32
Q

Acid amino acids (negative)

A
  • Aspartic acid

- Glutamic acid

33
Q

Polar (no charged) aminoacids

A
  • Asparagine
  • Glutamine
  • Serine
  • Threonine
  • Tyrosine
34
Q

Non polar amino acids (hydrophobic)

A
  • Alanine
  • Valine
  • Proline
  • Phenylalanine
  • Leucine
  • Isoleucine
  • Methionine
  • Tryptophan
  • Glycine
  • Cysteine
35
Q

Essential amino acids

A
  • Arginine
  • Hystidine
  • Isoleucine
  • Leucine
  • Lysine
  • Methionine
  • Phenylalanine
  • Threonine
  • Triptophan
  • Valine