amylase

Question 1

Found mainly in ____ and
slightly in the skeletal muscles, small intestines, and
fallopian tubes

Answer 1

pancreas and salivary glands

Question 2

Catalyzes breakdown of

Answer 2

starch and glycogen with products glucose, maltose, dextrin

Question 3

it is the smallest enzyme and can be filtered by the __

Answer 3

glomerulus

Question 4

Significant in the diagnosis of

Answer 4

acute pancreatitis and
salivary gland lesions.

Question 5

AMS requires activators such as

Answer 5

calcium and chloride

Question 6

Diagnostic significance

Answer 6

• Acute pancreatitis
• Salivary gland lesion like mumps and parotitis
• Intraabdominal diseases
• Renal insufficiency
• Diabetic ketoacidosis
• Macroamylasemia

Question 7

AMS combines with Ig to form a large complex that is not filtrerable by the glomerulus

Answer 7

Maacroamylasemia

Question 8

predominates in urine

Answer 8

P-type (P1, P2, P3)

Question 9

predominates in serum

Answer 9

S-type (S1, S2, S3):

Question 10

migrates faster than the P-type

Answer 10

S-type (S1, S2, S3):

Question 11

S-type AMS is inhibited by __

Answer 11

wheat germ lectin

Question 12

To measure S-AMS and P-AMS, determine the ____ in the presence and absence of lectin.

Answer 12

T-AMS

Question 13

Measures the disappearance of starch substrate

Answer 13

Amyloclastic

Question 14

Measures the appearance of the product

Answer 14

Saccharogenic

Question 15

Measures the increasing color from production of product coupled with chromogenic dye

Answer 15

Chromogenic

Question 16

Coupling of several enzyme systems to monitor amylase activity

Answer 16

Continuous monitoring

Question 17

measures the time required to reach the chromic point

Answer 17

Amyloclastic or Iodometric Method

Question 18

fast disappearance means high AMS activity

Answer 18

Amyloclastic or Iodometric Method

Question 19

starch substrate is hydrolyzed by AMS to its constituent carbohydrate molecule that has
reducing property like sugars

Answer 19

Saccharogenic

Question 20

employs a starch substrate with chromogenic dye that forms an insoluble dye-substrate complex

Answer 20

Chromolytic / Chromogen Labelled Substrate Method (Colorimetric)

Question 21

the higher the color intensity of the soluble dye-substrate
complex, the higher the AMS activity

Answer 21

Chromolytic / Chromogen Labelled Substrate Method (Colorimetric)

Question 22

measures the change in absorbance of
NAD+ at 340 nm at pH=6.9

Answer 22

Coupled Enzyme Reaction or Continuous
Monitoring method

Question 23

Coupled Enzyme Reaction or Continuous Monitoring method

Substrate

Answer 23

maltotetrase or maltopentoase

Question 24

Coupled Enzyme Reaction or Continuous Monitoring method

Indicator reaction

Answer 24

conversion of NAD to NADH monitored at 340 nm

Question 25

sources of error

Answer 25

* Acute pancreatitis with hyperlipemia * morphine and other opiates for pain relief may **falsely elevate** AMS * oxalates and citrates may **falsely decrease** the level of AMS

Question 26

# sources of error what factor would falsely elevate AMS

Answer 26

morphine and other opiates for pain relie

Question 27

# sources of error what factor would falsely decrease AMS

Answer 27

oxalates and citrates

Question 28

reference range of serum

Answer 28

28-100 U/L

Question 28

reference range of urine

Answer 28

1-15 U/hour

Question 29

what sample should not be used

Answer 29

hemolyzed

Question 30

concentration

Answer 30

x 9227

Question 31

The substrate, ___reacts directly 3 with α-amylase in the sample and does not require the presence of ancillary enzymes.

Answer 31

2-chloro-4-nitrophenyl-maltotrioside (CNPG)

Question 32

The release of ____ from the substrate and the resulting absorbance increase per minute at 405 nm is directly related to the α-amylase activity in the sample.

Answer 32

2-chloro-4-nitrophenol (CNP)