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Micro Lab > Anaerobes > Flashcards

Anaerobes Flashcards

1
Q

What should always accompany anaerobe cultures

A

aerobe cultures/ specials

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2
Q

Bacteroides fragilis: GS, colony morphology

A

GNR
grey, glistening

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3
Q

Clostridium perfringens: GS, hemolysis

A

GPR, boxy, boxcar, double zone of hemolysis

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4
Q

Prevotella: morphology, UV

A

black colonies
red under UV

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5
Q

Fusobacterium: GS, UV

A

GNR, pointy
green/ chartreuse under UV

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6
Q

What other organism can also be chartreuse under UV

A

C. diff

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7
Q

What two organisms are aerotolerant

A

Propionobacterium/ cuty
actinomyces

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8
Q

What organisms should you workup on anaerobic bench

A

Bacteriodes fragilis, Clostridium, Prevotella, Fusobacterium, actinomyces, propionobacterium/ cuty

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9
Q

If there are 3 or more anaerobes on the same specimen, what should you pull out to fully ID

A

B. fragilis
Clostridium

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10
Q

What are the anaerobic conditions

A

85% N2
10% CO2
5% H2

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11
Q

Actinomyces colony morphology

A

molar tooth, sulfur granules

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12
Q

If an organism is R to Colistin and S to Vanc it is likely

A

GP

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13
Q

If an organism is S to Colistin and R to Vanc it is likely

A

GN

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14
Q

What is an exception for an organism that is R to vanc

A

lactobacillus- GP but R to vanc

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15
Q

What organism is S to SPS

A

Peptococcan

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16
Q

What is the best source of specimen for anaerobic culture

A

deep tissue or aspirate

17
Q

What specimen should be rejected in anaerobes

A

skin swab

18
Q

What are the agars used in anaerobes

A

CDCANA
CNA
BBE- B. fragilis
LKV- Prevotella, B. fragilis

19
Q

What extra media do joints get and why

A

CMG- grows propionobacterium after 2 weeks

20
Q

Propionobacterium: biochemicals, O2 requirements, growth length

A

Catalase and indole +, aerotolerant, 2 weeks to grow

21
Q

What 2 organisms are strict anaerobes

A

Clostridium and fusobacterium

22
Q

B. urealyticum morphology

A

pits the agar

23
Q

Breadcrumb or ground glass

A

Fusobacterium

24
Q

Black pigmented colonies

A

Prevotella

25
Q

Molar tooth

A

Actinomyces

26
Q

Double zone of hemolysis

A

clostridium perfringens

Micro Lab (5 decks)

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