Anaerobes

Question 1

indicator in anaerobe jar

Answer 1

impregnated with methylene blue
blue = presence of oxygen
colorless = anaerobic conditions exist

Question 2

what is PRAS

Answer 2

Prereduced anaerobically sterilized

Question 3

Fusobacterium and C. diff present which color under the UV light?

Answer 3

Chartreuse green

Question 4

Gram stain is positive, but no growth on culture. what could be the reason?

Answer 4

patient in antibiotics
wrong plate
no anaerobic condition

Question 5

Disc pattern for Bacteroides

Answer 5

Colistin R
Kanamycin R
Vancomycin R

Question 6

Disc pattern for Prevotella

Answer 6

Colistin S
Kanamycin R
Vancomycin R

Question 7

Disc pattern for Porphyromonas

Answer 7

Colistin R
Kanamycin R
Vancomycin S

Question 8

Disc pattern for Fusobacterium

Answer 8

Colistin S
Kanamycin S
Vancomycin R

Question 9

Disc pattern for Capnocytophaga

Answer 9

Colistin S
Kanamycin S
Vancomycin R

Question 10

what organisms are positive for Nagler test?

Answer 10

C. perfringens
C. sordelli
C. bifermantens
C. barati

Question 11

Indole is detected by

Answer 11

p-dimethylaminocinnamaldehyde to produce blue-green color

Question 12

what organisms are positive for Indole

Answer 12

Cutibacterium acne (P.acne)
Peptostreptococcus asaccharolyticus

Question 13

what organism gives false negative indole?

Answer 13

Clostridiums

Question 14

gram stain, disc pattern, and nitrate for Veillonella

Answer 14

GNC
Colistin S
Kanamycin S
Vancomycin R
Nitrate +

Question 15

Usual disc pattern for GP organisms

Answer 15

Colistin R
Vancomycin S

Question 16

Disc pattern for P. asaccharolyticus

Answer 16

Colistin R
Kanamycin S
Vancomycin S

Question 17

Disc pattern for P. anaerobius

Answer 17

Colistin R
Kanamycin R
Vancomycin S

Question 18

Media used for C. diff

Answer 18

CCFA
Cycloserine-Cefoxitin Fructose Agar

Question 19

What are the aerotolerant organisms

Answer 19

C. histolyticum
C. teritum
P. acne

Question 20

What organism has sulfur granules and have molar tooth colony morphology?

Answer 20

Actinomyces

Question 21

What is GNC anaerobes

Answer 21

Veillonella

Question 22

Microaerophilic anaerobe GNR?

Answer 22

Capnocytophaga

Question 23

dog bone or forked end morphology

Answer 23

Bifidobacterium

Question 24

which organism causes pseudomembranous colitis and antibiotic-associated diarrhea

Answer 24

C. diff

Question 24

What are anaerobe GPC

Answer 24

Peptostreptococcus

Question 25

GPR chain catalase -

Answer 25

Lactobacillus

Question 26

GPR curved aerotolerant catalase + indole +

Answer 26

P. acne

Question 27

Swarmer Clostridiums

Answer 27

C. septicum C. tetani

Question 28

GPR tennis racket shapes potent neurotoxins (neuroparalytic disease)

Answer 28

C. botulinum

Question 29

Double zone hemolysis alpha exotoxin gas gangrene motility -

Answer 29

C. perfringens

Question 30

what organisms gives brick red fluorescence under the UV light

Answer 30

Prevotella Porphyromonas Veillonella

Question 31

Lactobacillus resembles

Answer 31

C. tertium

Question 32

Fusobacterium clinical disease

Answer 32

necrotizing faciitis

Question 33

potent neurotoxin (neuroparalytic disease) non-acidic vegetables, mushrooms, canned food, honey

Answer 33

C. botulinum

Question 34

GNR anaerobes Growth on BBE/LKV (black pigment on BBE) Disc pattern RRR causes pelvic inflammation

Answer 34

Bacteroides fragilis

Question 35

which media does Mycoplasma pneumoniae grow, gives what morphology, and clinical disease?

Answer 35

PPLO (pleural pneumonia-like organism) agar spherical, grainy Walking pneumonia

Question 36

which media does Mycoplasma hominis grow, gives what morphology, and inhabits?

Answer 36

A8 media Fried egg morphology inhabits the vagina, but can cause urethritis

Question 37

Which media does Ureaplasma urealyticum grow, gives what morphology, and found in?

Answer 37

A8 media Very tiny colonies normal flora in vagina, but can cause urethritis and infertility

Question 38

what organism causes trisma?

Answer 38

Lock jaw C. tetani