Anaerobic GPRs Flashcards Preview

Liz's Microbiology Module 2 > Anaerobic GPRs > Flashcards

Flashcards in Anaerobic GPRs Deck (56):
1

Propionibacterium acnes
- Normal flora

Skin (contaminant)

2

Propionibacterium acnes
- GLC

GLC = aP

3

Propionibacterium acnes
- Diseases or infections it's involved in

CNS shunt infections, endocarditis, prosthetic joint infections, eye
- Found in blood and CSF cultures after 5 days

4

Bifidobacterium
- Normal flora

Common fecal flora

5

Bifidobacterium
- Gram stain

Y-shaped GPR
- Non-sporeforming

6

Lactobacillus
- Normal flora

GI and vaginal flora

7

Lactobacillus
- Gram stain

Non-sporeforming GPR

8

Actinomyces
- Gram stain

Often branching GPRs
- Non-sporeforming

9

Actinomyces israelii
- Colonial morphology

ROUGH/SLOW growth
- Molar tooth 5-14 days or longer

10

Actinomyces israelii
- Gram stain

Branching GPRs
- Non-sporeforming
- Chunky in broth

11

Actinomyces israelii
- Diseases or infections it's involved in

- Actinomycosis → "Lumpy Jaw"
- Sulfur granules from drainage

12

Mobiluncus
- Gram stain

Motile, sligthly curved GPR
- Non-sporeforming

13

Mobiluncus
- Diseases or infections it's involved in

Non-specific vaginitis or bacterial vaginosis

14

Eubacterium
- Diseases or infections it's involved in

Rarely cause disease b/c it's a beneficial bacterium

15

Eubacterium
- Normal flora

Intestinal tract

16

Eggerthella lenta
- Normal flora

Intestinal tract

17

Three types of spores

- Terminal
- Central
- Subterminal

18

Most clinical isolates of anaerobic gram positive sporeforming rods are ____

Clostridium spp

19

Clostridium perfringens
- Gram stain

GPR
- Sporeforming
- Often stains Gram negative on a direct smear

20

Clostridium perfringens
- What are the reactions w/ milk, lecithinase, lipase, and reverse CAMP test?

- Milk: proteolytic (curd/digest)
- Lecithinase: positive on EYA
- Lipase: negative on EYA
- Reverse CAMP: positive

21

Clostridium perfringens
- Colonial morphology

Double zone beta hemolysis

22

Clostridium perfringens
- 2 major diseases

- Gas gangrene
- Food poisoning

23

Gas gangrene (myonecrosis)
- Most common cause
- Toxins
- What happens

- Clostriidum
- Cytotoxins destroy cells/tissues
- Gas, bullae, discoloration

24

Crepitant (anaerobic) cellulitis
- What is NOT involved?

Muscle and facia

25

Clostridium perfringens food poisoning is found in what foods?

Meats and gravy

26

Clostridium ramosum
- Normal flora

GI

27

Clostridium ramosum
- Gram stain

Virgina rail fence and palisading; terminal spores rarely seen

28

Clostridium septicum
- Colonial morphology

Swarms; spreading colonies; slight beta hemolysis

29

Clostridium septicum
- Gram stain

Citron-shaped GPRs

30

Clostridium septicum
- 2 diseases

- Gas gangrene
- Bacteremia associated w/ malignancy (colon cancer, leukemia or lymphoma)

31

Clostridium septicum
- For diseases, are the mortality rates high or low and can therapy make a difference?

- High mortality rates
- Early recognition and proper therapy can make a signficant difference in mortality

32

Clostridium sordellii
- Gram stain

Chaining GPR, w/ lots of free spores

33

Clostridium sordellii
- Colonial morphology

Very irregular colonies

34

Clostridium tetani
- Gram stain

GPRs w/ LARGE terminal spores

35

Clostridium tetani
- Colonial morphology

Irregular shaped colonies, "course ground glass"

36

Tetanus
- Another name/what does it cause
- Infection or intoxication?

- "Lockjaw"
- Intoxication

37

Clostridium botulinum
- 3 infections

- Foodborne
- Infant botulism (aka Floppy Baby Syndrome)
- Wound botulism

38

Foodborne C. botulinum
- Found in what foods
- Infection or intoxication
- What occurs from disease
- Treatment

- Found in home canned foods
- Intoxication
- Occurs from descending "flaccid paralysis"
- Treatment is an antitoxin

39

Infant botulism caused by C. botulinum
- Another name
- What food is implicated
- Infection or intoxication

- aka Floppy Baby Syndrome
- Found in honey
- Infection

40

Clostridium sporogenes
- Lipase reaction

Lipase +

41

Clostridium sporogenes
- Gram stain

GPR w/ subterminal and free spores

42

Clostridium sporogenes
- Colonial morphology

Can swarm (filamentous, medusa head colonies)

43

Clostridium difficile
- Normal flora

Intestinal flora

44

Clostridium difficile
- Colonial morphology

Large colonies, irregular edge and shape, raised yellowish to grey-white with a COARSE "CIS"

45

Clostridium difficile
- GLC

GLC = a...IC

46

Clostridium difficile causes an _____ _____ diarrhea

Antibiotic associated → pseudomembraneous colitis

47

What is true about C. difficile diseases's mortality?

Bloody diarrhea can be fatal

48

Why does C. difficile disease potentially serious threat to immunocompromised patients?

Spores on hands, bed sheets, surfaces in hospital environment

49

Clostridium difficile
- 2 major toxins

- Toxin A = enterotoxin
- Toxin B = cytotoxin

50

2 ways to identify Clostridium difficile

- Rapid real-time PCR
- Gold standard toxigenic culture for C. diff (using cycloserine, cerfoxitin, fructose agar → CCFA) and ID (but too slow)

51

Rapid real-time PCR assays for which of Clostridium difficile's toxins

Toxin B

52

Clostridium clostridioforme
- Gram stain

GPR that more commonly stains Gram negative; tapered ends
- Football shaped

53

Propionibacterium acnes
- Colonial morphology

May be aerotolerant

54

Aerotolerant Clostridium form spores under ____ conditions

Anaerobic
- Grows better anaerobically compared w/ growth in CO2

55

3 examples of aerotolerant Clostridium

- C. carnis
- C. histolyticum
- C. tertium

56

Mobiluncus
- Normal flora

Vaginal