analyzing proteins and DNA

Question 1

used to separate organelles based on size and density

Answer 1

ultracentrifuge

Question 2

separates proteins based on charge

Answer 2

ion exchange chromatography

Question 3

chromatography that separates proteins based on size

Answer 3

gel-filtration chromatography

Question 4

separates proteins based on attraction

Answer 4

affinity chromatography

Question 5

creates an even charge so that things can be divided based on size

Answer 5

SDS page

Question 6

separates proteins based on isoelectric point and then size

Answer 6

2D gels/electrophoresis

Question 7

allows for the identification of unknown proteins

Answer 7

mass spectroscopy

Question 8

used to identify the presence of protein resolved by SDS page and MW could be determined

Answer 8

western blott

Question 9

can be used to identify a protein that can be in complex with a known protein

Answer 9

immunoprecipitation

Question 10

detects the presence of antigen; used when quantification of an antigen from a slurry of protein (like a blood sample) is desired

Answer 10

direct ELISA

Question 11

detects the presence of antibodies and is used for HIV testing

Answer 11

Indirect ELISA

Question 12

allows for the detection and quantification of antigen

Answer 12

used for pregnancy tests

Question 13

tells us about shape and anatomic distances within the molecule (must be able to crystallize the protein)

Answer 13

X-ray chromatography

Question 14

can analyze protein in solution (limited by size)

Answer 14

NMR

Question 15

separates DNA particles based on size

Answer 15

Agarose gel

Question 16

DNA copy of mRNA

Answer 16

cDNA clones

Question 17

used to analyze the presence and location of genes-cytogenetics

Answer 17

FISH

Question 18

used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude (can detect alterations)

Answer 18

PCR

Question 19

can be used for paternity tests

Answer 19

STR

Question 20

similar to determining length of DNA; rough way to check if DNA comes from a certain individual

Answer 20

RFLP

Question 21

used to track inheritances of diseases in families

Answer 21

SNPs

Question 22

used to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome

Answer 22

DNA microarray chip