Antibiotics MOAs

Question 1

Beta lactams (which are…?

Answer 1

Form covalent bonds with PBPs to inhibit final cross-link formation

Question 2

Glycopeptides

Answer 2

Inhibit release of the building block unit from the cell membrane, preventing addition to the growing end of the peptidoglycan.

Question 3

Quinolones (which are…?)

Answer 3

Inhibit DNA gyrase to prevent bacterial DNA supercoiling

Question 4

Metronidazole

Answer 4

Form reactive intermediates that inhibit DNA synthesis (damage DNA)

Question 5

Rifampicin

Answer 5

Inhibit RNA polymerase to prevent mRNA trancription

Question 6

Tetracyclines (which are…?)

Answer 6

Binds to 30S subunit of ribosome and actively competes with tRNA for A site to inhibit binding.

Question 7

Aminoglycosides (which are…?)

Answer 7

Binds to 30S subunit of ribosome and causes misreading of codon on mRNA resulting in non-functional protein production.

Question 8

Chloramphenicol

Answer 8

Inhibits transpeptidation (growing peptide chain attached to P site is transferred to AA attached to tRNA in A site)

Question 9

Macrolides (which are…?)

Answer 9

Binds to 50S subunit and inhibits translocation (where tRNA in A site is moved to P site)

Question 10

Sulphonamides

Answer 10

Inhibit synthesis of folate by inhibiting dihydrofolate synthetase

Question 11

Trimethoprim

Answer 11

Inhibit use of folate by inhibiting dihydrofolate reductase