Antibiotics Sensitivity Testing

Question 1

Method for antibiotics sensitivity testing

Answer 1

. Dilution method
.Disc diffusion
.E test (combination of upper 2)
. Automated method (DEAD)

Question 2

Dilution method

Answer 2

. 0.5 Mc Farlands = 1.5 × 10⁸ Cfu /ml
.micro broth - 96 wells , save time and medium.
.macro broth - test tube used, requires time

Question 3

Disc Diffusion method /Kirby Bauer Disc Diffusion method

Answer 3

.lawn culture used incuabte , dispense, incubate again at 37°c
.CAMHA agar used.
🥰 can measure Zone of Inhibitons
😡can’t measure Mic i.e can’t quantity

Question 4

Stoke’s Disc Diffusion method

Answer 4

.Control tell validity

Question 5

E- test ( Epsilomtere)

Answer 5

this meter measure Mic