Antibodies To Things

Question 1

Ds-DNA (double stranded DNA)

Answer 1

MOST highly specific for SLE

Lupus nephritis

Question 2

What Ab can you order with a high sensitivity for SLE?

Answer 2

Ds-DNA

• a/w high specificity for SLE
• predicts lupus nephritis
• correlates with disease severity

Question 3

What antibody do you order which is a/w lupus nephritis in SLE?

Answer 3

Double-stranded DNA or Anti-Sm

• also anti-Smith does as well
• ds-DNA has a high specificity for SLE, predicts disease severity, correlates with early/severe disease

Question 4

Anti-Sm

Answer 4

A/w lupus nephritis, highly specific for SLE

*So is anti-dsDNA

Question 5

Anti-rRNP (U1 RNP)

Answer 5

R-RNP - Highly specific for neuropsychiatric LE *per Jain

U1 RNP - Highly specific for MCTD *per Hocker

Question 6

What antibody do you test if you are suspicious for neuropsychiatric LE?

Answer 6

Anti-rRNP (U1RNP)

Question 7

Anti-Ro (SSA)

Anti-La (SSB)

Answer 7

SJOGRENS!(Most strong association)
SCLE
Neonatal lupus/congenital heart block

Question 8

What antibodies do you order to check for SCLE

Answer 8

Anti Ro(SSA)/La(SSB)
*also a/w congenital heart block

Question 9

Anti-SSA,Anti-SSB is the same Ab as what?

Answer 9

Anti-Ro/Anti-La

*checks for SCLE/neonatal lupus/congenital heart block/ AND Sjogrens

Question 10

Antibody to check for neonatal lupus?

Answer 10

Anti-Ro/La (same thing as SSA/SSB)

Question 11

Strongest Ab a.w Sjogrens

Answer 11

Anti-Ro, Anti-La

• MOST SPECIFIC -Anti fodrin
• which are also a/w SCLE/neonatal lupus

Question 12

What two diseases is anti-Ro/La a/w?

Answer 12

SJOGRENS (strong) (sidenote* anti-fodrin is most specific)

SCLE/neonatal lupus

Question 13

Anti-U1RNP

Answer 13

MCTD

*also can see in SCLE but mostly MCTD

Question 14

Anti-histone

Answer 14

Drug induced SLE

Question 15

Antibody indicating drug induced SLE/SCLE

Answer 15

Anti-his tone

Question 16

Anti-KU

Answer 16

SLE with polymyositis

Question 17

Antibody for drug induced polymyositis

Answer 17

Anti-KU

Question 18

Anti-ssDNA

Answer 18

Increased risk of SLE in DLE

Question 19

What antibody would you order in a DLE patient to assess their possible risk for SLE?

Answer 19

Anti-ssDNA

Question 20

Anti-dsDNA

Anti-ssDNA

Answer 20

Anti-dsDNA is most specific test for SLE

Anti-ssDNA is risk of SLE within a DLE patient

Question 21

Anti-C1q

Answer 21

Severe SLE

*URTICARIAL VASCULITIS

Question 22

Test for antiphospholipid syndrome in SLE

Answer 22

Anti-cardiolipin

Question 23

Anti-Fodrin

Answer 23

Most SPECIFIC for Sjogrens!!!

*also strong is anti-Ro/La

Question 24

Which Ab a/w systemic disease and lymphoma in Sjogrens

Answer 24

Anti Ro (other Ab associated w Sjogrens are Ro/La and Fodrin)

Question 25

Anti-Scl-70

Answer 25

Same thing as DNA-topoisomerase I (seen in systemic sclerosis)

Question 26

Anti-Scl-70 is the same this as what antigen

Answer 26

DNA topoisomerase I. (Seen in severe systemic sclerosis)

Question 27

Ab most strongly a/w diffuse skin disease in systemic sclerosis

Answer 27

Anti-Scl-70 (DNA topoisomerase I)

Question 28

Anti-centromere

Answer 28

CREST

Question 29

Ab a/w CREST

Answer 29

Anti-centromere

Question 30

Anti-RNA Polymerase III

Answer 30

Severe skin and KIDney disease in systemic sclerosis *RNA polymerase the third, is part of a long family line with lots of KIDS, severe punishment for bad kids

Question 31

Marker for severe renal and skin disease in systemic sclerosis

Answer 31

RNA polymerase III

Question 32

Anti-fibrillarin

Answer 32

same thing as U3RNP, marker for pulmonary HTN in systemic sclerosis *remember U1RNP is marker of SLE and also MCTD

Question 33

Marker for pulmonary HTN in systemic sclerosis

Answer 33

Anti-fibrillarin (same thing as U3RNP)

Question 34

U3RNP

Answer 34

Same thing as anti-fibrillarin *marker for internal organ involvement in systemic sclerosis like pulmonary HTN

Question 35

Name 4 antibodies you would check in evaluation a patient for systemic sclerosis besides ANA

Answer 35

``` Anti- Centrome Fibrillarin Scl-70 RNA polymerase III ```

Question 36

Anti-155 kDa/anti-Se

Answer 36

Cancer in dermatomyositis, amyopathic DM

Question 37

Which Ab is a/w cancer in DM or amyopathic DM?

Answer 37

Anti-155 kDa (also called anti-Se)

Question 38

Anti-Jo

Answer 38

Antisynthetase syndrome (DM w raynauds, mechanics hands, pulm fibrosis)

Question 39

Ab for antisynthetase syndrome (in DM)

Answer 39

Anti-Jo

Question 40

Anti-SRP

Answer 40

Cardiac involvement in DM (SRP*CPR**)

Question 41

Anti-Mi2

Answer 41

Hallmark skin lesions in DM, a/w good prognosis

Question 42

Which Ab is a/w cardiac findings in DM

Answer 42

Anti-SRP

Question 43

Which Ab is a/w typical DM skin lesions and carries a good prognosis?

Answer 43

Anti-Mi2

Question 44

Anti-p40

Answer 44

Anti-juvenille DM with calcinosis

Question 45

Juvenile DM with calcinosis Ab

Answer 45

Anti-p140

Question 46

Anti-PL-7, Anti-PL12

Answer 46

Antisynthetase syndrome

Question 47

Name all of the Ab you can think of a/w antisynthetase syndrome

Answer 47

Anti-Jo Anti PL-7 Anti PL-12 EJ/OJ

Question 48

The hallmark of antisynthetase syndrome is the presence of serum antibodies directed against which type of proteins?

Answer 48

Histidyl tRNA synthetases

Question 49

Mi-2 is a good prognosis in which diseasE?

Answer 49

DM

Question 50

Which antibody carries a good prognosis in DM?

Answer 50

Mi2!

Question 51

Ab for Cardiac involvement in DM

Answer 51

Anti-SRP

Question 52

Anti-SRP

Answer 52

Cardiac involvement in DM

Question 53

What cells are the substrate for indirect immunoflouresence in an ANA test?

Answer 53

Hep2 cancer cell line

Question 54

What autoimmune disorder is least likely to have a positive ANA?

Answer 54

DERMATOMYOSITIS *only positive in 40-65% *SLE has 99% positive ANA

Question 55

Which antibody correlates with a lupus band test from SUN PROTECTED skin?

Answer 55

Anti-dsDNA

Question 56

Antibody a\w amyopathic DM interstiatial lung disease

Answer 56

Anti-MDA-5 *BOARDS Q!!!!!!