Antibody Antigen Testing

Question 1

What is immunodiagnostics

Answer 1

Detects protein or part of pathogen

Question 2

How to culture viruses

Answer 2

Need to culture in living cell as they are obligate pathogens

Question 3

What does an ELISA detect

Answer 3

Antigens or Antibodies

Question 4

What is done to viruses before PCR and why

Answer 4

They must be reverse transcribed as they have an RNA genome

Question 5

2 examples of animal-side tests to detect pathogen

Answer 5

Lateral flow
Latex Agglutination

Question 6

What is detected by an Indirect ELISA

Answer 6

Test for antibodies

Question 7

What is detected by sandwich ELISA

Answer 7

Detects antigen (IgM most useful)

Question 8

What does IgM indicate

Answer 8

Active/ very recent infection

Question 9

What type of hypersensitivity is used in TB tuberculin test

Answer 9

IV

Question 10

How does interferon gamma test for TB work

Answer 10

take blood sample in heparin tube, challenge blood cells in test tubes with tuberculin from chickens and cows, if animal has been previously exposed the white blood cells with react by producing interferon gamma which you can measure

Question 11

What is a latex agglutination test

Answer 11

The latex agglutination reaction is a variation on the clumping ability observed when a sample containing the specific antigen is mixed with an antibody coated on the surface of latex particles.