AS Booklet 3- Viruses, Cell Structure, Cell Transport and Mitosis Flashcards
What are Viruses?
Viruses are non-living, acellular. Viruses have no nucleus, organelles, cell-surface membrane and no cytoplasm.
What is the structure of a virus?
Capsid (usually protein), usually always genetic material either DNA or RNA. An envelope (mostly lipids) and attachment proteins.
How do viruses replicate?
Non-living so they attach to specific host cell’s cell-surface membrane via attachment proteins.
Usually viruses inject genetic info into host cell. Viral DNA used as template to create viral proteins and eventually whole viruses which often release via lysis of the host cell.
What is the cell structure of Prokaryotes?
Murein cell wall
Cell-surface membrane
Circular DNA molecule free in cytoplasm, not relation to a protein.
70s ribosomes.
Cytoplasm.
OCCASIONALLY: capsule surrounding cell wall, one+ plasmids, one+ flagella.
What is the cell structure of Eukaryotes?
Cell-surface membrane. Nucleus. Linear DNA related to protein (histones). Membrane-bound organelles. 80s ribosomes Cytoplasm.
What are the differences and similarities between the cell structures of prokaryotic cells and eukaryotic cells?
Differences: 70s vs 80s ribosomes. No organelles vs organelles No nucleus vs
Similarities: Cell-surface membrane.
What are the named organelles of the eukaryotic cell?
Nucleus, Ribosome, Mitochondrium, SER and RER, Golgi body and apparatus, Lysosome, Chloroplast.
What is the structure and function of the Nucleus of the eukaryotic cell?
Ribosomes, RER, pernicular space (in between inner and outer nuclear membrane, which forms nuclear envelope), nuclear pore, chromatin, nucleolus.
Contains DNA (genetic information) and more than one or more nucleoli. Controls protein synthesis and therefore development and function of cell.
What is the structure and function of the Ribosome of the eukaryotic cell?
Small, made up of protein and rRNA (small and large subunit) Appear in cytoplasm alone or on RER.
Used in protein synthesis, joins amino acids together.
What is the structure and function of the SER and RER of the eukaryotic cell?
RER: has ribosomes on it that produce secretory enzymes. These secretory enzymes go to Golgi body for modification/packaging.
SER: doesn’t have ribosomes, involved in production and transport of lipids.
What is the structure and function of the Golgi body and apparatus of the eukaryotic cell?
Golgi apparatus is flattened membrane sacs just like SER/RER.
Adds carbohydrates to proteins from RER, forming glycoproteins.
Packages proteins/glycoproteins into Golgi vesicles for secretion.
Produces lysosomes that contain lysozymes (hydrologic enzymes).
What is the structure and function of the Mitochondria of the eukaryotic cell?
Circular DNA. Matrix. Inter membrane space. Enzymes. Inner membrane. Outer membrane. Cristal. 80s ribosomes. Function is the place where respiration takes place.
What is the structure and function of the Lysosomes of the eukaryotic cell?
Membrane sacs containing hydrolytic enzymes. Enzymes are kept separately so as not to destroy the cell.
Formed by Golgi Apparatus.
Functions:
Digestion of material from phagocytosis. Fuse with vesicles and release hydrolytic enzymes to digest the material and then this is deposited outside of the cell (lysosome fuses with membrane to release it).
Organelles that don’t work anymore/are non-functional are engulfed digested.
Release of hydrolytic enzymes outside of cell.
What is the structure and function of the Chloroplast in eukaryotic plant cells?
Lipid droplets. Circular DNA. Outer membrane. Inner membrane. Stroma. Granum. Thylakoid. Membrane-bound AND free 80s ribosomes. Starch grains.
What is the structure of a eukaryotic plant cell?
Murein Cell wall. Cell-surface membrane. RER and SER. Golgi Apparatus. Nucleolus. Nucleus. Chromatin. Large vacuole. Cytosol. Mitochondria. Plasmodesmata- fine strands that connect to adjacent cells.
Give a description of one type of cell from a multicellular organism?
An epithelial cell from the lining of the ileum.
Microvili, RER, SER, Golgi Apparatus, Cytoplasm, Nucleus, Nuclear membrane, 80s Ribosomes, cell-surface membrane, mitochondria.
What are the definitions of tissues, organs and systems?
Tissues: groups of similar cells performing specific functions, with a common origin.
Organs: different tissues, which have specific functions.
Systems: consists of two or more organs working together.
What is the process of cell fractionation and ultracentrifugation?
- Cells homogenised (by grinding) to release stuff within cell membrane and put into ice cold isotonic buffer solution, prevents damage to organelles by osmosis, pH or temperature change, enzymes hydrolysing them due to increase in temp = increase in KE.
- Suspension is put into centrifuge at low speed.
- Sediment and supernatant are divided.
- Supernatant mixed at medium speed.
- New sediment and supernatant separated.
- Supernatant mixed at high speed.
- Sediment and supernatant separated, supernatant is soluble protein.
Have to spin at low speed-high speed because at high speed immediately, all organelles would sink.
What is Electron Microscopy?
The use of a beam of electrons to magnify a sample.
What are the advantages and disadvantages of Electron Microscopy?
Advantages:
• There is a greater resolution because electrons have shorter wavelengths of light.
Disadvantages:
• Vacuum is required so live samples can’t be seen.
• Preparation involving staining is more complicated than light microscopy + can result in artefacts.
• Thin sections must be prepared.
Images aren’t in colour.
How does a transmission electron microscope (TEM) work?
- Uses a beam of electrons focused by electromagnets.
- Specimen must be thing, stained by electron-dense substances like heavy metal salts.
- These substances deflect electrons and pattern made by remaining electrons of the beam passing through specimen converted to image.
How does a scanning electron microscope (SEM) work?
- Specimen coated with thin film of heavy metal like gold.
- Electron beam is scanned to and fro across specimen.
- Electrons reflected from surface produce image seen.
What are the advantages and disadvantages of a TEM?
Advantages: • Higher resolution than SEM. • Can see internal structures (proteins, nucleic acids etc). Disadvantages: • Isn't 3D. • No colour.
What are the advantages and disadvantages of an SEM?
Advantages:
• Surfaces of structure shown.
• 3D effect.
• Thicker sections can be examined than in TEM.
Disadvantages:
• Lower resolution than TEM.
• Only surface (of cross-section) can be viewed.