Assay of Acetaminophen Tablets Using HPLC

Question 1

Separation technique in which the components of a sample are distributed between the two phases

Answer 1

chromatography

Question 2

2 phases of chromatography

Answer 2

1. stationary phase
2. mobile phase

Question 3

layer or coating on the supporting medium which INTERACTS WITH THE ANALYTES

Answer 3

stationary phase

Question 4

CARRIES THE SOLUTE across the stationary phase

Answer 4

mobile phase

Question 5

Classification according to nature of mobile and stationary phase

Answer 5

1. Normal
2. Reverse

Question 6

Polar compounds are highly adsorbed

Answer 6

normal

Question 7

Non-polar compounds are highly adsorbed

Answer 7

reverse

Question 8

Normal
- Stationary Phase: ______________
- Mobile Phase: __________________

Answer 8

SP: polar
MP: non-polar

Question 9

Reverse
- Stationary Phase: ______________
- Mobile Phase: __________________

Answer 9

SP: non-polar
MP: polar

Question 10

Normal chromatography

___________________ (polar/non-polar) phase would be eluted FIRST

Answer 10

non-polar

Question 11

Normal chromatography

___________________ (polar/non-polar) phase would be eluted LAST

Answer 11

polar

Question 12

Reverse chromatography

___________________ (polar/non-polar) phase would be eluted FIRST

Answer 12

polar

Question 13

Reverse chromatography

___________________ (polar/non-polar) phase would be eluted LAST

Answer 13

non-polar

Question 14

Mechanisms of separation

Answer 14

1. adsorption
2. partition
3. ion-exchange

Question 15

Separation involves COMPETING INTERACTIONS between adsorption at the stationary phase and dissolution in the mobile phase

Answer 15

adsorption

Question 16

Particles are SEPARATED ON THE COMPONENTS of the solvent system

Answer 16

partition

Question 17

Separation is based mainly on DIFFERENCES IN THE ION EXCHANGE AFFINITIES of the sample components

Answer 17

ion-exchange

Question 18

Types of chromatography useful in qualitative and quantitative analyses in USP procedures

Answer 18

1. paper chrom
2. TLC
3. column chrom
4. gas chrom
5. HPLC

Question 19

HPLC
- Stationary Phase: ______________
- Mobile Phase: __________________

Answer 19

SP: solid
MP: liquid solvent system

Question 20

uses same type of solvent system

Answer 20

isocratic

Question 21

technique of continuously changing the solvent composition during the chromatographic run

Answer 21

gradient elution

Question 22

[T/F] adsorption, partition, or ion-exchange can all be used as the mechanism of separation for HPLC

Answer 22

TRUE

Question 23

organic/aqueous solvents are used depending on the analysis

Answer 23

mobile phase

Question 24

used to remove dissolved air from the mobile phase

Answer 24

degassing unit

Question 25

used to deliver the mobile phase at constant flow

Answer 25

solvent delivery pump

Question 26

used to store std soln or sx soln

Answer 26

sample vial

Question 27

introduce std soln or real sx to the HPLC column

Answer 27

injector

Question 28

used to keep temperature constant

Answer 28

column oven

Question 29

used to separate each compound contained in the sample

Answer 29

column

Question 30

used to detect the eluted compounds from the column

Answer 30

detector

Question 31

apparatus in which the signal from the detector is processed and the chromatogram is displayed

Answer 31

workstation

Question 32

The term column includes _________________, _______________________________, and _________________________, packed with a stationary phase

Answer 32

stainless steel, lined stainless steel, and polymeric columns

Question 33

The _______________________________ of the column affects the separation, and therefore typical column dimensions are included in the individual monograph

Answer 33

length and inner diameter

Question 34

a GRAPHICAL REPRESENTATION of the detector response, concentration of analyte in the effluent, or other quantity used as a measure of effluent concentration versus effluent volume or time

Answer 34

chromatogram

Question 35

time elapsed between the injection of the sample and the appearance of the maximum peak response of the eluted sample

Answer 35

retention time (tR)

Question 36

a measure of the concentration of the compound it represents

Answer 36

area under a peak

Question 37

C8H9NO2

Answer 37

acetaminophen

Question 38

acceptance criteria of acetaminophen tablets

Answer 38

90.0% - 110.0% on the dried basis